PUBLICATION
Combined toxic effects of fludioxonil and triadimefon on embryonic development of zebrafish (Danio rerio)
- Authors
- Wang, Y., Xu, C., Wang, D., Weng, H., Yang, G., Guo, D., Yu, R., Wang, X., Wang, Q.
- ID
- ZDB-PUB-200212-5
- Date
- 2020
- Source
- Environmental pollution (Barking, Essex : 1987) 260: 114105 (Journal)
- Registered Authors
- Keywords
- Aquatic toxicology, Enzymatic activity, Gene expression, Joint toxicity
- MeSH Terms
-
- Animals
- Dioxoles/toxicity*
- Ecosystem
- Embryo, Nonmammalian
- Embryonic Development
- Larva
- Oxidative Stress
- Pyrroles/toxicity*
- Superoxide Dismutase
- Triazoles/toxicity*
- Water Pollutants, Chemical/toxicity*
- Zebrafish/embryology*
- PubMed
- 32041085 Full text @ Environ. Pollut.
Citation
Wang, Y., Xu, C., Wang, D., Weng, H., Yang, G., Guo, D., Yu, R., Wang, X., Wang, Q. (2020) Combined toxic effects of fludioxonil and triadimefon on embryonic development of zebrafish (Danio rerio). Environmental pollution (Barking, Essex : 1987). 260:114105.
Abstract
Pesticides scarcely exist as individual compounds in the water ecosystem, but rather as mixtures of multiple chemicals at relatively low concentrations. In this study, we aimed to explore the mixture toxic effects of fludioxonil (FLU) and triadimefon (TRI) on zebrafish (Danio rerio) by employing different toxicological endpoints. Results revealed that the 96-h LC50 values of FLU to D. rerio at multiple developmental stages ranged from 0.055 (0.039-0.086) to 0.61 (0.33-0.83) mg L-1, which were less than those of TRI ranging from 3.08 (1.84-5.96) to 9.75 (5.99-14.78) mg L-1. Mixtures of FLU and TRI exerted synergistic effects on embryonic zebrafish. Activities of total superoxide dismutase (T-SOD) and catalase (CAT) were markedly altered in most of the individual and pesticide mixture treatments compared with the control. The expressions of 16 genes involved in oxidative stress, cellular apoptosis, immune system and endocrine system displayed that embryonic zebrafish were affected by the individual pesticides and their mixtures, and greater variations of four genes (ERɑ, Tnf, IL and bax) were found when exposed to pesticide mixtures compared with their individual compounds. Therefore, more studies on mixture toxicities among different pesticides should be taken as a priority when evaluating their ecological risk.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping