ZFIN ID: ZDB-PUB-200208-5
Cell and tissue manipulation with ultrashort infrared laser pulses in light-sheet microscopy
de Medeiros, G., Kromm, D., Balazs, B., Norlin, N., G√ľnther, S., Izquierdo, E., Ronchi, P., Komoto, S., Krzic, U., Schwab, Y., Peri, F., de Renzis, S., Leptin, M., Rauzi, M., Hufnagel, L.
Date: 2020
Source: Scientific Reports   10: 1942 (Journal)
Registered Authors: Leptin, Maria, Peri, Francesca
Keywords: none
MeSH Terms:
  • Animals
  • Drosophila melanogaster/physiology
  • Imaging, Three-Dimensional/methods
  • Infrared Rays
  • Lasers
  • Microscopy*
  • Zebrafish/physiology
PubMed: 32029815 Full text @ Sci. Rep.
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ABSTRACT
Three-dimensional live imaging has become an indispensable technique in the fields of cell, developmental and neural biology. Precise spatio-temporal manipulation of biological entities is often required for a deeper functional understanding of the underlying biological process. Here we present a home-built integrated framework and optical design that combines three-dimensional light-sheet imaging over time with precise spatio-temporal optical manipulations induced by short infrared laser pulses. We demonstrate their potential for sub-cellular ablation of neurons and nuclei, tissue cauterization and optogenetics by using the Drosophila melanogaster and zebrafish model systems.
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