PUBLICATION

Cell and tissue manipulation with ultrashort infrared laser pulses in light-sheet microscopy

Authors
de Medeiros, G., Kromm, D., Balazs, B., Norlin, N., Günther, S., Izquierdo, E., Ronchi, P., Komoto, S., Krzic, U., Schwab, Y., Peri, F., de Renzis, S., Leptin, M., Rauzi, M., Hufnagel, L.
ID
ZDB-PUB-200208-5
Date
2020
Source
Scientific Reports   10: 1942 (Journal)
Registered Authors
Leptin, Maria, Peri, Francesca
Keywords
none
MeSH Terms
  • Animals
  • Drosophila melanogaster/physiology
  • Imaging, Three-Dimensional/methods
  • Infrared Rays
  • Lasers
  • Microscopy*
  • Zebrafish/physiology
PubMed
32029815 Full text @ Sci. Rep.
Abstract
Three-dimensional live imaging has become an indispensable technique in the fields of cell, developmental and neural biology. Precise spatio-temporal manipulation of biological entities is often required for a deeper functional understanding of the underlying biological process. Here we present a home-built integrated framework and optical design that combines three-dimensional light-sheet imaging over time with precise spatio-temporal optical manipulations induced by short infrared laser pulses. We demonstrate their potential for sub-cellular ablation of neurons and nuclei, tissue cauterization and optogenetics by using the Drosophila melanogaster and zebrafish model systems.
Genes / Markers
Figures
Figure Gallery
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes