PUBLICATION

Expression patterns of pcbp gene family members during zebrafish embryogenesis

Authors
Wang, J., Guo, X., Wang, D., Yang, S.
ID
ZDB-PUB-200203-2
Date
2020
Source
Gene expression patterns : GEP   35: 119097 (Journal)
Registered Authors
Yang, Shulan
Keywords
Development, Expression pattern, Poly(C)-Binding proteins family, Zebrafish
MeSH Terms
  • Animals
  • Brain/embryology
  • Brain/metabolism
  • Gastrointestinal Tract/embryology
  • Gastrointestinal Tract/metabolism
  • Gene Expression Regulation, Developmental*
  • Organogenesis*
  • RNA-Binding Proteins/genetics*
  • RNA-Binding Proteins/metabolism
  • Retina/embryology
  • Retina/metabolism
  • Zebrafish
  • Zebrafish Proteins/genetics*
  • Zebrafish Proteins/metabolism
PubMed
32007595 Full text @ Gene Expr. Patterns
Abstract
The poly(C)-binding protein (PCBP) family members belong to a subtype of RNA-binding proteins that are ubiquitously expressed with diverse functions. In mammals, PCBP family, also known as hnRNP E family, is composed of four proteins, namely PCBP1, PCBP2, PCBP3 and PCBP4. So far, no study has been documented on the physiological roles of each member in vertebrate development. Here we analysed the spatiotemporal expression patterns of zebrafish (Danio rerio) pcbp2 (identical to pcbp1 and pcbp2 in mammals), pcbp3 and pcbp4 at various stages of zebrafish embryonic development by whole-mount in situ hybridization. Our results revealed that all pcbp genes are maternally expressed, especially pcbp2, which is strongly expressed from the embryogenetic stage to larva. The expression patterns of PCBP members are similar to each other at the very early developmental stage sharing with common strong expression in the intestine, otic vesicle, retina and brain of zebrafish. Subsequently, the messenger RNAs of PCBP members are gradually constrained and highly expressed in intestines of the larvae. Collectively, our study figured out the expression pattern of each PCBP member in diverse organogenesis during embryo development, indicating that PCBP members may play predominant roles in the development of neural and digestive systems to maintain their normal physiological functions. Moreover, the similar expression patterns at the developmental stages and organ types among this family suggest that the aberrant expression of these genes would lead to the neural or intestinal diseases.
Genes / Markers
Figures
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Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes