PUBLICATION

Fucoidan from Laminaria japonica exerts antitumor effects on angiogenesis and micrometastasis in triple-negative breast cancer cells

Authors
Hsu, W.J., Lin, M.H., Kuo, T.C., Chou, C.M., Mi, F.L., Cheng, C.H., Lin, C.W.
ID
ZDB-PUB-200201-17
Date
2020
Source
International journal of biological macromolecules   149: 600-608 (Journal)
Registered Authors
Cheng, Chia-Hsiung
Keywords
Angiogenesis, Fucoidan, Invasion, Metastasis, Triple-negative breast cancer
MeSH Terms
  • Apoptosis/drug effects
  • Cell Line, Tumor
  • Cell Proliferation/drug effects
  • Female
  • Gene Expression Regulation, Neoplastic/drug effects
  • Humans
  • Laminaria/chemistry*
  • Mitogen-Activated Protein Kinase Kinases/genetics
  • NF-kappa B/genetics
  • Neoplasm Micrometastasis
  • Neovascularization, Pathologic/drug therapy*
  • Neovascularization, Pathologic/pathology
  • Polysaccharides/chemistry
  • Polysaccharides/pharmacology*
  • Signal Transduction/drug effects
  • Transcription Factor AP-1/genetics
  • Triple Negative Breast Neoplasms/drug therapy*
  • Triple Negative Breast Neoplasms/pathology
  • Xenograft Model Antitumor Assays
PubMed
32004612 Full text @ Int. J. Biol. Macromol.
Abstract
Fucoidan is a fucose-rich polysaccharide that has gained attention for its various anticancer properties. However, the effect and underlying mechanism of fucoidan on triple-negative breast cancer (TNBC) are still unknown. Herein, we investigated the anticancer potential of fucoidan from Laminaria japonica. We found that fucoidan showed modest antiproliferative activity against TNBC cells, while it effectively reduced migratory and invasive capacities. Mechanistically, fucoidan suppressed activation of MAPK and PI3K followed by inhibition of AP-1 and NF-κB signaling in TNBC. Additionally, fucoidan downregulated expressions of proangiogenic factors in TNBC cells, and fucoidan blocked tumor-elicited tube formation by human umbilical vascular endothelial cells (HUVECs). We also observed that fucoidan blocked tumor adhesion and invasion towards HUVECs. Surprisingly, fucoidan robustly suppressed tube formation on HUVECs. Moreover, fucoidan inhibited in vivo angiogenesis and micrometastasis in a transgenic zebrafish model. Together, L. japonica fucoidan exhibits potent antitumor effects by its attenuation of invasiveness and proangiogenesis in TNBC.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping