PUBLICATION

Effects of prednisolone on behavior and hypothalamic-pituitary-interrenal axis activity in zebrafish

Authors
Xin, N., Jiang, Y., Liu, S., Zhou, Y., Cheng, Y.
ID
ZDB-PUB-200112-2
Date
2020
Source
Environmental Toxicology and Pharmacology   75: 103325 (Journal)
Registered Authors
Keywords
Behavior, HPI axis, Neurotransmitter, Prednisolone, Zebrafish
MeSH Terms
  • Zebrafish Proteins
  • Behavior, Animal/drug effects*
  • Zebrafish
  • Animals
  • Water Pollutants, Chemical/toxicity*
  • Hypothalamo-Hypophyseal System/drug effects
  • Prednisolone/toxicity*
PubMed
31924570 Full text @ Environ. Toxicol. Pharmacol.
Citation
Xin, N., Jiang, Y., Liu, S., Zhou, Y., Cheng, Y. (2020) Effects of prednisolone on behavior and hypothalamic-pituitary-interrenal axis activity in zebrafish. Environmental Toxicology and Pharmacology. 75:103325.
Abstract
Prednisolone is a synthetic glucocorticoid used clinically for treating allergies, inflammation, and autoimmune diseases. Long-term prednisolone use has been shown to have negative effects on physiology and mood. We aimed to study the pharmacology and toxicology of glucocorticoid-like drugs by investigating behavioral and hypothalamic-pituitary-interrenal (HPI) axis effects in a zebrafish model. Zebrafish embryos 24 h post fertilization were exposed to 25 μM prednisolone. Their behavior was investigated 5 days post fertilization (dpf), and their HPI axis-related activity and related neurotransmitter levels were investigated 3, 4, 5, and 6 dpf. The behavior results showed that exposure to prednisolone resulted in decreased autonomic activity and low sensitivity to light. qRT-PCR and ELISA results showed decreased activity of the HPI axis and increased secretion of dopamine and serotonin after exposure to prednisolone. This study provides us with new insights into understanding the effects of glucocorticoids on the HPI axis.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
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