PUBLICATION
A benzothiazole-based ratiometric fluorescent probe for detection of formaldehyde and its applications for bioimaging
- Authors
- Hao, Y., Zhang, Y., Zhang, A., Sun, Q., Zhu, J., Qu, P., Chen, S., Xu, M.
- ID
- ZDB-PUB-200110-19
- Date
- 2019
- Source
- Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy 229: 117988 (Journal)
- Registered Authors
- Keywords
- Benzothiazole, Fluorescent probe, Formaldehyde, Imaging, Ratiometric
- MeSH Terms
-
- Molecular Imaging/methods*
- Zebrafish/metabolism*
- Humans
- Spectrometry, Fluorescence/methods*
- Animals
- HeLa Cells
- Fluorescent Dyes/chemistry*
- Benzothiazoles/chemistry*
- Formaldehyde/analysis*
- PubMed
- 31918154 Full text @ Spectrochim Acta A Mol Biomol Spectrosc.
Citation
Hao, Y., Zhang, Y., Zhang, A., Sun, Q., Zhu, J., Qu, P., Chen, S., Xu, M. (2019) A benzothiazole-based ratiometric fluorescent probe for detection of formaldehyde and its applications for bioimaging. Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy. 229:117988.
Abstract
We presented a new benzothiazole-based fluorescent probe for ratiometric sensing of formaldehyde. Upon treatment with formaldehyde, the alkylamine-functionalized probe can be converted to its aldehyde analogue via the target-mediated 2-aza-Cope rearrangement, which led to significant shifts in both absorption (from 392 to 452 nm) and emission (from 492 to 552 nm) bands. The sensing mechanism was confirmed by HPLC, UV/Vis and fluorescence spectroscopy. The probe is capable of sensing formaldehyde under physiological conditions with high selectivity over potentially competing biological analytes. The probe also displayed sensitive ratiometric fluorescence response (up to 35.7 fold) for formaldehyde with a low limit detection of 0.58 μM. Furthermore, the probe was successfully employed for ratiometric imaging of formaldehyde in living cells as well as in zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping