In vivo single-cell lineage tracing in zebrafish using high-resolution infrared laser-mediated gene induction microscopy
- He, S., Tian, Y., Feng, S., Wu, Y., Shen, X., Chen, K., He, Y., Sun, Q., Li, X., Xu, J., Wen, Z., Qu, J.Y.
- eLIFE 9: (Journal)
- Registered Authors
- Wen, Zilong, Xu, Jin
- developmental biology, regenerative medicine, stem cells, zebrafish
- MeSH Terms
- Cell Lineage*
- Microscopy, Confocal*
- Single-Cell Analysis/instrumentation
- Single-Cell Analysis/methods*
- 31904340 Full text @ Elife
He, S., Tian, Y., Feng, S., Wu, Y., Shen, X., Chen, K., He, Y., Sun, Q., Li, X., Xu, J., Wen, Z., Qu, J.Y. (2020) In vivo single-cell lineage tracing in zebrafish using high-resolution infrared laser-mediated gene induction microscopy. eLIFE. 9:.
Heterogeneity broadly exists in various cell types both during development and at homeostasis. Investigating heterogeneity is crucial for comprehensively understanding the complexity of ontogeny, dynamics, and function of specific cell types. Traditional bulk-labeling techniques are incompetent to dissect heterogeneity within cell population, while the new single-cell lineage tracing methodologies invented in the last decade can hardly achieve high-fidelity single-cell labeling and long-term in-vivo observation simultaneously. In this work, we developed a high-precision infrared laser-evoked gene operator heat-shock system, which uses laser-induced CreERT2 combined with loxP-DsRedx-loxP-GFP reporter to achieve precise single-cell labeling and tracing. Invivo study indicated that this system can precisely label single cell in brain, muscle and hematopoietic system in zebrafish embryo. Using this system, we traced the hematopoietic potential of hemogenic endothelium (HE) in the posterior blood island (PBI) of zebrafish embryo and found that HEs in the PBI are heterogeneous, which contains at least myeloid unipotent and myeloid-lymphoid bipotent subtypes.
Genes / Markers
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Engineered Foreign Genes
Errata and Notes