PUBLICATION
Spatio-temporal expression and distribution of collagen VI during zebrafish development
- Authors
- Tonelotto, V., Trapani, V., Bretaud, S., Heumüller, S.E., Wagener, R., Ruggiero, F., Bonaldo, P.
- ID
- ZDB-PUB-191231-6
- Date
- 2019
- Source
- Scientific Reports 9: 19851 (Journal)
- Registered Authors
- Bretaud, Sandrine, Ruggiero, Florence
- Keywords
- none
- MeSH Terms
-
- Animals
- Reverse Transcriptase Polymerase Chain Reaction
- Phylogeny
- Collagen Type I/classification
- Collagen Type I/genetics*
- Larva/genetics*
- Larva/growth & development
- Zebrafish Proteins/classification
- Zebrafish Proteins/genetics*
- In Situ Hybridization
- Spatio-Temporal Analysis
- Muscle, Skeletal/embryology
- Muscle, Skeletal/growth & development
- Gene Expression Regulation, Developmental*
- Skin/embryology
- Skin/growth & development
- Collagen Type VI/classification
- Collagen Type VI/genetics*
- Animals, Genetically Modified
- Zebrafish/embryology
- Zebrafish/genetics*
- Zebrafish/growth & development
- PubMed
- 31882701 Full text @ Sci. Rep.
Citation
Tonelotto, V., Trapani, V., Bretaud, S., Heumüller, S.E., Wagener, R., Ruggiero, F., Bonaldo, P. (2019) Spatio-temporal expression and distribution of collagen VI during zebrafish development. Scientific Reports. 9:19851.
Abstract
Collagen VI (ColVI) is an extracellular matrix (ECM) protein involved in a range of physiological and pathological conditions. Zebrafish (Danio rerio) is a powerful model organism for studying vertebrate development and for in vivo analysis of tissue patterning. Here, we performed a thorough characterization of ColVI gene and protein expression in zebrafish during development and adult life. Bioinformatics analyses confirmed that zebrafish genome contains single genes encoding for α1(VI), α2(VI) and α3(VI) ColVI chains and duplicated genes encoding for α4(VI) chains. At 1 day post-fertilization (dpf) ColVI transcripts are expressed in myotomes, pectoral fin buds and developing epidermis, while from 2 dpf abundant transcript levels are present in myosepta, pectoral fins, axial vasculature, gut and craniofacial cartilage elements. Using newly generated polyclonal antibodies against zebrafish α1(VI) protein, we found that ColVI deposition in adult fish delineates distinct domains in the ECM of several organs, including cartilage, eye, skin, spleen and skeletal muscle. Altogether, these data provide the first detailed characterization of ColVI expression and ECM deposition in zebrafish, thus paving the way for further functional studies in this species.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping