PUBLICATION
Zinc chloride influences embryonic development, growth, and Gh/Igf-1 gene expression during the early life stage in zebrafish (Danio rerio)
- Authors
- Horie, Y., Yonekura, K., Suzuki, A., Takahashi, C.
- ID
- ZDB-PUB-191226-33
- Date
- 2019
- Source
- Comparative biochemistry and physiology. Toxicology & pharmacology : CBP 230: 108684 (Journal)
- Registered Authors
- Keywords
- Ecotoxicity, Growth hormone, Insulin-like growth factor, OECD test guideline no. 210, Zinc
- MeSH Terms
-
- Insulin-Like Growth Factor I/metabolism
- Zygote*/growth & development
- Zygote*/metabolism
- Growth Hormone/metabolism
- Water Pollutants, Chemical/toxicity*
- Zinc/toxicity*
- Embryonic Development
- Lethal Dose 50
- Zinc Compounds/toxicity*
- Animals
- Zebrafish*/growth & development
- Zebrafish*/metabolism
- Chlorides/toxicity*
- Larva/growth & development*
- Larva/metabolism
- PubMed
- 31874288 Full text @ Comp. Biochem. Physiol. C Toxicol. Pharmacol.
Citation
Horie, Y., Yonekura, K., Suzuki, A., Takahashi, C. (2019) Zinc chloride influences embryonic development, growth, and Gh/Igf-1 gene expression during the early life stage in zebrafish (Danio rerio). Comparative biochemistry and physiology. Toxicology & pharmacology : CBP. 230:108684.
Abstract
Although zinc is an essential element for organisms, excess zinc exposure is harmful. We assessed the possible negative influence of zinc (Zn) on the freshwater fish Danio rerio during its early life stage by using Organization for Economic Cooperation and Development test guideline no. 210. Lethality of Zn after hatching occurred in a concentration dependent manner. The LC50 and lowest observed effect concentration of mortality values in the present toxicity assay were 2.31 mg/L (95% confidence limit: 1.81-3.05) and 1.5 mg/L, respectively. These values were close to the reported concentration recorded in aquatic environments. Growth inhibition was observed at 15 and 30 days post-hatching with Zn exposure of 1.5 mg/L. In general, the growth hormone (Gh)/insulin-like growth factor-I (Igf-1) axis is important for growth in fishes, and Zn exposure induced a significant reduction of igf-1 expression at the concentration that caused growth inhibition. These findings suggest that the observed growth inhibition was induced by the suppression of igf-1 expression. In addition, these results suggest that by examining gene expression on the Gh/Igf-1 axis, it may be possible to predict growth suppression by chemical exposure.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping