PUBLICATION
Water Extract of Yamato Tachibana (Citrus tachibana) Induces Food Intake in Adult and Larval Zebrafish
- Authors
- Yamada, Y., Chensom, S., Yonemoto, H., Nakayama, H., Zang, L., Nishimura, N., Mishima, T., Shimada, Y.
- ID
- ZDB-PUB-191219-17
- Date
- 2019
- Source
- Journal of Medicinal Food 23(1): 65-71 (Journal)
- Registered Authors
- Keywords
- appetite, nutritional composition, weight gain, zebrafish
- MeSH Terms
-
- 3T3-L1 Cells
- Animals
- Blood Glucose/analysis
- Body Weight/drug effects
- Cholesterol/blood
- Citrus/chemistry*
- Eating/drug effects*
- Fruit/chemistry*
- Japan
- Larva
- Mice
- Plant Extracts/pharmacology*
- Triglycerides/blood
- Water
- Zebrafish/genetics*
- PubMed
- 31851855 Full text @ J. Med. Food
Citation
Yamada, Y., Chensom, S., Yonemoto, H., Nakayama, H., Zang, L., Nishimura, N., Mishima, T., Shimada, Y. (2019) Water Extract of Yamato Tachibana (Citrus tachibana) Induces Food Intake in Adult and Larval Zebrafish. Journal of Medicinal Food. 23(1):65-71.
Abstract
Yamato Tachibana (Tachibana; Citrus tachibana) is an endemic fruit and represents one of the oldest citrus species in Japan; it is grown in the Mie Prefecture. It has been attracting attention for its cultural heritage and unique scent. To evaluate biological activities of Tachibana, we fed several parts of the Tachibana fruit (whole fruit, pulp [albedo and segment wall], and flavedo) to adult zebrafish and found that Tachibana increased body weight and plasma triglycerides besides increasing overall food intake. We then created a simple fluorescence-based feeding assay using dried rotifer sheets and larval zebrafish (6 days postfertilization) to screen the various extracts of Tachibana parts. We found that water extracts of Tachibana pulp increased feeding volume in zebrafish. Although citrus species are believed to prevent obesity and obesity-associated diseases in general, our findings showed that water extracts of Tachibana increase food intake in zebrafish and lead to an increase in body weight. We suggest that Tachibana might reverse appetite loss in lean populations and may prove beneficial in aiding fish cultivation.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping