PUBLICATION
Physiological and transcriptional effects of mixtures of environmental estrogens, androgens, progestins and glucocorticoids in zebrafish
- Authors
- Willi, R., Castiglioni, S., Salgueiro Gonzalez, N., Furia, N., Mastroianni, S., Faltermann, S., Fent, K.
- ID
- ZDB-PUB-191213-9
- Date
- 2019
- Source
- Environmental science & technology 54(2): 1092-1101 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Androgens
- Animals
- Estrogens
- Glucocorticoids
- Progestins
- Water Pollutants, Chemical*
- Zebrafish*
- PubMed
- 31829580 Full text @ Env. Sci. Tech.
Citation
Willi, R., Castiglioni, S., Salgueiro Gonzalez, N., Furia, N., Mastroianni, S., Faltermann, S., Fent, K. (2019) Physiological and transcriptional effects of mixtures of environmental estrogens, androgens, progestins and glucocorticoids in zebrafish. Environmental science & technology. 54(2):1092-1101.
Abstract
Fish are exposed to mixtures of different class steroids but ecotoxicological implications are not sufficiently known. Here we systematically analyze effects of different combinations of steroid mixtures in zebrafish embryos to assess their joint activities on physiology and transcriptional alterations of steroid-specific target genes at 96 and 120 hours post fertilization. In binary mixtures of clobetasol propionate (CLO) with estradiol (E2) or androstenedione (A4) each steroid exhibited its expression profile. This was also the case in mixtures of five, eight and 13 different class steroids in exposure concentrations of 10-10000 ng/L. Transcriptional expression of most genes in different mixtures was steroid-specific with the exception of genes encoding aromatase (cyp19b), sulfatase (sult2st3) and cyp2k22 that were induced by androgens, progestins and glucocorticoids. Marked alterations occurred for sult2st3 in binary mixtures of CLO+E2 and CLO+A4. Glucocorticoids increased heart rate and muscle contractions. In mixtures containing estrogens induction of the cyp19b transcript occurred at 10 ng/L and protc from the anticoagulation system at 100 ng/L. Our study demonstrates that steroids can act independently in mixtures; the sum of individual steroid profiles is expressed. However, some genes, including cyp19b, sult2st3 and cyp2k22, are regulated by several steroids. This joint effect on different pathways may be of concern for fish development.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping