PUBLICATION
A short period of dark-adaptation is sufficient to generate light-induced photoreceptor degeneration in pigmented zebrafish
- Authors
- Khan, A.S., Friemel, T.D., Grillo, S.L., Stella, S.L.
- ID
- ZDB-PUB-191204-10
- Date
- 2020
- Source
- Neuroreport 31: 22-28 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Animals
- Dark Adaptation
- Disease Models, Animal*
- Female
- Light/adverse effects*
- Male
- Nerve Regeneration/physiology
- Photoreceptor Cells, Vertebrate/pathology*
- Retinal Degeneration/etiology
- Retinal Degeneration/pathology*
- Zebrafish
- PubMed
- 31789762 Full text @ Neuroreport
Citation
Khan, A.S., Friemel, T.D., Grillo, S.L., Stella, S.L. (2020) A short period of dark-adaptation is sufficient to generate light-induced photoreceptor degeneration in pigmented zebrafish. Neuroreport. 31:22-28.
Abstract
Light-induced retinal degeneration (LIRD) models are used to recapitulate the pathologies of retinal diseases that affect photoreceptors. Current LIRD models use a dark-adaptation period of 7-14 days followed by high-intensity light exposure. The purpose of this study was to determine whether photoreceptor damage and death would occur in pigmented zebrafish using a short period of dark-adaptation. Zebrafish were dark-adapted for 24 h and then exposed to constant high-intensity light for 48 h. Immunohistochemical analysis was performed on vertical retinal sections to assess damage and apoptosis. Photoreceptors exhibited structural damage, apoptosis, and cell loss after 24 and 48 h of light exposure as previously reported in studies using 7-14 day dark-adaption. Also, photoreceptors lost following light damage were regenerated after 28 days. These results suggest that a short period of dark-adaptation is sufficient for a LIRD model in pigmented zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping