PUBLICATION
Vitamin C inhibits lipid deposition through GSK-3β/mTOR signaling in the liver of zebrafish
- Authors
- Liu, D., Gu, Y., Pang, Q., Han, Q., Li, A., Wu, W., Zhang, X., Shi, Q., Zhu, L., Yu, H., Zhang, Q.
- ID
- ZDB-PUB-191130-15
- Date
- 2019
- Source
- Fish physiology and biochemistry 46(1): 383-394 (Journal)
- Registered Authors
- Keywords
- Danio rerio, GSK-3β, Lipid deposition, Liver, Vitamin C, mTOR
- MeSH Terms
-
- Animals
- Ascorbic Acid*
- Glycogen Synthase Kinase 3 beta/metabolism*
- Lipid Metabolism
- Lipids
- Liver
- Signal Transduction
- TOR Serine-Threonine Kinases/metabolism*
- Zebrafish/physiology*
- Zebrafish Proteins/metabolism*
- beta Catenin
- PubMed
- 31782040 Full text @ Fish Physiol. Biochem.
Citation
Liu, D., Gu, Y., Pang, Q., Han, Q., Li, A., Wu, W., Zhang, X., Shi, Q., Zhu, L., Yu, H., Zhang, Q. (2019) Vitamin C inhibits lipid deposition through GSK-3β/mTOR signaling in the liver of zebrafish. Fish physiology and biochemistry. 46(1):383-394.
Abstract
In this study, the mechanism that VC inhibits lipid deposition through GSK-3β/mTOR signaling was investigated in the liver of Danio rerio. The results indicated that 0.5- and 1.0-g/kg VC treatments activated mTOR signaling by inhibiting GSK-3β expression. The mRNA expression of FAS, ACC, and ACL, as well as the content of TG, TC, and NEFA, was decreased by 0.5- and 1.0-g/kg VC treatments. Moreover, to confirm GSK-3β playing a key role in regulating TSC2 and mTOR, GSK-3β RNA was interfered and the activity of GSK-3β was inhibited by 25- and 50-mg/L LiCl treatments, respectively. The results indicated that GSK-3β inactivation played a significant role in inducing mTOR signaling and inhibiting lipid deposition. VC treatments could induce mTOR signaling by inhibiting GSK-3β, and mTOR further participated in regulating lipid deposition by controlling lipid profile in the liver of zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping