PUBLICATION
Acidified water impairs the lateral line system of zebrafish embryos
- Authors
- Lin, L.Y., Hung, G.Y., Yeh, Y.H., Chen, S.W., Horng, J.L.
- ID
- ZDB-PUB-191112-12
- Date
- 2019
- Source
- Aquatic toxicology (Amsterdam, Netherlands) 217: 105351 (Journal)
- Registered Authors
- Horng, Jiun-Lin
- Keywords
- Acidic, Environment, Hair cell, Mechanotransducer channel, Scanning ion-selective electrode technique, Zebrafish
- MeSH Terms
-
- Animals
- DNA-Binding Proteins/genetics
- Ecosystem
- Embryo, Nonmammalian/drug effects*
- Embryo, Nonmammalian/metabolism
- Embryo, Nonmammalian/ultrastructure
- Embryonic Development/drug effects*
- Fresh Water/chemistry*
- Gene Knockdown Techniques
- Hair Cells, Auditory/drug effects
- Hair Cells, Auditory/ultrastructure
- Hydrochloric Acid/administration & dosage
- Hydrogen-Ion Concentration
- Lateral Line System/drug effects*
- Mechanotransduction, Cellular/drug effects
- Transcription Factors/genetics
- Vacuolar Proton-Translocating ATPases/genetics
- Water Pollutants, Chemical/toxicity*
- Zebrafish*/embryology
- Zebrafish Proteins/genetics
- PubMed
- 31711007 Full text @ Aquat. Toxicol.
Citation
Lin, L.Y., Hung, G.Y., Yeh, Y.H., Chen, S.W., Horng, J.L. (2019) Acidified water impairs the lateral line system of zebrafish embryos. Aquatic toxicology (Amsterdam, Netherlands). 217:105351.
Abstract
Acidification of freshwater ecosystems is recognized as a global environmental problem. However, the influence of acidic water on the early stages of freshwater fish is still unclear. This study focused on the sublethal effects of acidic water on the lateral line system of zebrafish embryos. Zebrafish embryos were exposed to water at different pH values (pH 4, 5, 7, 9, and 10) for 96 (0-96 h post-fertilization (hpf)) and 48 h (48∼96 hpf). The survival rate, body length, and heart rate significantly decreased in pH 4-exposed embryos during the 96-h incubation. The number of lateral-line neuromasts and the size of otic vesicles/otoliths also decreased in pH 4-exposed embryos subjected to 96- and 48-h incubations. The number of neuromasts decreased in pH 5-exposed embryos during the 96-h incubation. Alkaline water (pH 9 and 10) did not influence embryonic development but suppressed the hatching process. The mechanotransducer channel-mediated Ca2+ influx was measured to reveal the function of lateral line hair cells. The Ca2+ influx of hair cells decreased in pH 5-exposed embryos subjected to the 48-h incubation, and both the number and Ca2+ influx of hair cells had decreased in pH 5-exposed embryos after 96 h of incubation. In addition, the number and function of hair cells were suppressed in H+-ATPase- or GCM2-knockdown embryos, which partially lost the ability to secrete acid into the ambient water. In conclusion, this study suggests that lateral line hair cells are sensitive to an acidic environment, and freshwater acidification could be a threat to the early stages of fishes.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping