PUBLICATION
A novel hepatic lectin of zebrafish Danio rerio is involved in innate immune defense
- Authors
- Yang, Q., Wang, P., Yang, S., Li, X., Zhang, X., Ji, G., Zhang, S., Wang, S., Li, H.
- ID
- ZDB-PUB-191108-12
- Date
- 2019
- Source
- Fish & shellfish immunology 98: 670-680 (Journal)
- Registered Authors
- Li, Hongyan
- Keywords
- C-type lectin, Danio rerio, Hepatic lectin, Innate immunity, Zebrafish
- MeSH Terms
-
- Amino Acid Sequence
- Gene Expression Regulation/immunology*
- Gram-Negative Bacteria/physiology
- Base Sequence
- Zebrafish/genetics*
- Zebrafish/immunology*
- Gram-Positive Bacteria/physiology
- Fish Proteins/chemistry
- Fish Proteins/genetics
- Fish Proteins/immunology
- Animals
- Immunity, Innate/genetics*
- Gene Expression Profiling/veterinary
- Fish Diseases/immunology*
- PubMed
- 31689552 Full text @ Fish Shellfish Immunol.
Citation
Yang, Q., Wang, P., Yang, S., Li, X., Zhang, X., Ji, G., Zhang, S., Wang, S., Li, H. (2019) A novel hepatic lectin of zebrafish Danio rerio is involved in innate immune defense. Fish & shellfish immunology. 98:670-680.
Abstract
ASGPR (asialoglycoprotein receptor, also known as hepatic lectin) was the first identified animal lectin, which participated in a variety of physiological processes. Yet its detailed immune functions are not well studied in lower vertebrates. After reporting a zebrafish hepatic lectin (Zhl), we identified a novel hepatic lectin (zebrafish hepatic lectin-like, Zhl-l) in zebrafish. The zhl-l was mainly expressed in liver in a tissue specific manner. And challenge with LPS/LTA induced a significant change of zhl-l expression. What's more, recombinant C-type lectin domain (rCTLD) of Zhl-l had the activity of agglutinating and binding to both Gram-negative and Gram-positive bacteria. It promoted the phagocytosis of bacteria by carp macrophages. Moreover, rCTLD could bind to insoluble lipopolysaccharide (LPS), lipoteichoic acid (LTA) and peptidoglycan (PGN) independent of Ca2+, which was inhibited by galactose. Interestingly, Zhl-l was located in the membrane, and its overexpression could upregulate the production of pre-inflammatory cytokines. Taken together, these results indicated that Zhl-l played a role in immune defense, and would provide further information to understand functions of C-type lectin family and the innate immunity in vertebrates.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping