PUBLICATION

A novel hepatic lectin of zebrafish Danio rerio is involved in innate immune defense

Authors
Yang, Q., Wang, P., Yang, S., Li, X., Zhang, X., Ji, G., Zhang, S., Wang, S., Li, H.
ID
ZDB-PUB-191108-12
Date
2019
Source
Fish & shellfish immunology   98: 670-680 (Journal)
Registered Authors
Li, Hongyan
Keywords
C-type lectin, Danio rerio, Hepatic lectin, Innate immunity, Zebrafish
MeSH Terms
  • Gram-Negative Bacteria/physiology
  • Animals
  • Fish Proteins/chemistry
  • Fish Proteins/genetics
  • Fish Proteins/immunology
  • Fish Diseases/immunology*
  • Amino Acid Sequence
  • Gene Expression Regulation/immunology*
  • Immunity, Innate/genetics*
  • Gene Expression Profiling/veterinary
  • Gram-Positive Bacteria/physiology
  • Base Sequence
  • Zebrafish/genetics*
  • Zebrafish/immunology*
(all 14)
PubMed
31689552 Full text @ Fish Shellfish Immunol.
Abstract
ASGPR (asialoglycoprotein receptor, also known as hepatic lectin) was the first identified animal lectin, which participated in a variety of physiological processes. Yet its detailed immune functions are not well studied in lower vertebrates. After reporting a zebrafish hepatic lectin (Zhl), we identified a novel hepatic lectin (zebrafish hepatic lectin-like, Zhl-l) in zebrafish. The zhl-l was mainly expressed in liver in a tissue specific manner. And challenge with LPS/LTA induced a significant change of zhl-l expression. What's more, recombinant C-type lectin domain (rCTLD) of Zhl-l had the activity of agglutinating and binding to both Gram-negative and Gram-positive bacteria. It promoted the phagocytosis of bacteria by carp macrophages. Moreover, rCTLD could bind to insoluble lipopolysaccharide (LPS), lipoteichoic acid (LTA) and peptidoglycan (PGN) independent of Ca2+, which was inhibited by galactose. Interestingly, Zhl-l was located in the membrane, and its overexpression could upregulate the production of pre-inflammatory cytokines. Taken together, these results indicated that Zhl-l played a role in immune defense, and would provide further information to understand functions of C-type lectin family and the innate immunity in vertebrates.
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