PUBLICATION
Regulation of SRF protein stability by an autophagy-dependent pathway
- Authors
- Luo, J., Jin, F.Q., Yin, M., Jin, Z.G.
- ID
- ZDB-PUB-191102-8
- Date
- 2019
- Source
- Biochemical and Biophysical Research Communications 521(2): 279-284 (Journal)
- Registered Authors
- Keywords
- Autophagy lysosome pathway, Degradation, Serum response factor (SRF)
- MeSH Terms
-
- Animals
- Autophagy*
- COS Cells
- Cells, Cultured
- Chlorocebus aethiops
- Culture Media, Serum-Free/pharmacology
- Cycloheximide/pharmacology
- Glycogen Synthase Kinase 3 beta/metabolism
- Humans
- Lysosomes/metabolism
- Protein Stability
- Rats
- Serum Response Factor/chemistry*
- Serum Response Factor/metabolism
- PubMed
- 31668369 Full text @ Biochem. Biophys. Res. Commun.
Citation
Luo, J., Jin, F.Q., Yin, M., Jin, Z.G. (2019) Regulation of SRF protein stability by an autophagy-dependent pathway. Biochemical and Biophysical Research Communications. 521(2):279-284.
Abstract
Serum response factor (SRF), a key transcription factor, plays an important role in regulating cell functions such as proliferation and differentiation. Most proteins are unstable, and protein stability is regulated through the ubiquitin-proteasome system (UPS) or the autophagy lysosome pathway (ALP). Whether SRF is degraded and what mechanisms control SRF protein stability remain unexplored. Western blot analyses of cells treated with cycloheximide (CHX), a protein synthesis inhibitor, showed that SRF was degraded in a time-dependent manner. Moreover, we observed that SRF undergoes autophagy-dependent destruction, which is accelerated by serum deprivation. Through bioinformatics screening, we found that SRF contains the GSK3β phosphorylation motif (T/SPPXS): SPDSPPRSDPT, which is conserved from zebrafish to humans. Serum deprivation stimulated GSK3β activation that then potentiates SRF degradation through the autophagy lysosome pathway. Since SRF is important for numerous cellular activities, our results suggest that the autophagy-dependent SRF degradation pathway may provide a new avenue to modulate SRF-mediated cell functions.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping