PUBLICATION
Ochratoxin A triggered intracerebral hemorrhage in embryonic zebrafish: Involvement of microRNA-731 and prolactin receptor
- Authors
- Wu, T.S., Lin, Y.T., Huang, Y.T., Yu, F.Y., Liu, B.H.
- ID
- ZDB-PUB-191102-42
- Date
- 2019
- Source
- Chemosphere 242: 125143 (Journal)
- Registered Authors
- Keywords
- Intracerebral hemorrhage, Ochratoxin A, Prolactin receptor a, Vascular defect, Zebrafish embryos, microRNA-731
- MeSH Terms
-
- Animals
- Cerebral Hemorrhage/chemically induced*
- Embryo, Nonmammalian/drug effects
- Embryo, Nonmammalian/physiology
- Erythrocytes/drug effects
- MicroRNAs
- Mycotoxins
- Ochratoxins/toxicity*
- Receptors, Prolactin/metabolism
- Water Pollutants, Chemical/toxicity*
- Zebrafish/embryology
- Zebrafish/physiology
- PubMed
- 31675585 Full text @ Chemosphere
Citation
Wu, T.S., Lin, Y.T., Huang, Y.T., Yu, F.Y., Liu, B.H. (2019) Ochratoxin A triggered intracerebral hemorrhage in embryonic zebrafish: Involvement of microRNA-731 and prolactin receptor. Chemosphere. 242:125143.
Abstract
Ochratoxin A (OTA), a mycotoxin widely found in foodstuffs, reportedly damages multiple brain regions in developing rodents, but the corresponding mechanisms have not been elucidated. In this study, zebrafish embryos at 6 h post fertilization (hpf) were exposed to various concentrations of OTA and the phenomenon associated with intracerebral hemorrhage was observed at 72 hpf. Exposure of embryos to OTA significantly increased their hemorrhagic rate in a dose-dependent manner. Large numbers of extravagated erythrocytes were observed in the midbrain/hindbrain areas of Tg(fli-1a:EGFP; gata1:DsRed) embryos following exposure to OTA. OTA also disrupted the vascular patterning, especially the arch-shaped central arteries (CtAs), in treated embryos. Histological analysis revealed a cavity-like pattern in their hindbrain ventricles, implying the possibility of cerebral edema. OTA-induced intracerebral hemorrhage and CtA vessel defects were partially reversed by the presence of miR-731 antagomir or the overexpression of prolactin receptor a (prlra); prlra is a downstream target of miR-731. These results suggest that exposure to OTA has a negative effect on cerebral vasculature development by interfering with the miR-731/PRLR axis in zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping