PUBLICATION

Endocrine and local signaling interact to regulate spermatogenesis in zebrafish: Follicle-stimulating hormone, retinoic acid and androgens

Authors
Crespo, D., Assis, L.H.C., van de Kant, H.J.G., de Waard, S., Safian, D., Lemos, M.S., Bogerd, J., Schulz, R.W.
ID
ZDB-PUB-191011-22
Date
2019
Source
Development (Cambridge, England)   146(21): (Journal)
Registered Authors
Bogerd, Jan, Schulz, Rüdiger W.
Keywords
11-ketotestosterone, RNA sequencing, Retinoic acid, Spermatogenesis, Zebrafish
Datasets
GEO:GSE116611
MeSH Terms
  • Male
  • Spermatocytes/physiology
  • Signal Transduction*
  • Feedback, Physiological
  • Androgens/physiology*
  • Tretinoin/physiology*
  • Spermatids/physiology
  • Animals
  • Gene Expression Regulation, Developmental
  • Cell Differentiation/genetics
  • Zebrafish
  • Spermatogonia/physiology
  • Busulfan/chemistry
  • Endocrine System/physiology*
  • Testis/physiology
  • Spermatogenesis*
  • Follicle Stimulating Hormone/physiology*
  • Mice
  • Retinoids/physiology
  • Transgenes
(all 20)
PubMed
31597660 Full text @ Development
Abstract
Retinoic acid (RA) is critical for mammalian spermatogonia differentiation, and stimulates Stra8 expression, a gene required for meiosis. Certain fish species, including zebrafish, have lost the stra8 gene. While RA still seems important for spermatogenesis in fish, it is not known which stage(s) respond to RA or if its effects are integrated into the endocrine regulation of spermatogenesis. In zebrafish, RA promoted spermatogonia differentiation, supported androgen-stimulated meiosis and reduced spermatocyte and spermatid apoptosis. Follicle-stimulating hormone (Fsh) stimulated RA production. Expressing a dominant-negative RA receptor variant in germ cells clearly disturbed spermatogenesis but meiosis and spermiogenesis still took place although sperm quality was low in 6 months-old adults. This condition also activated Leydig cells. Three months later, spermatogenesis apparently had recovered, but doubling of testis weight demonstrated hypertrophy, apoptosis/DNA damage among spermatids was high and sperm quality remained low. We conclude that RA signaling is important for zebrafish spermatogenesis but is not of critical relevance. Since Fsh stimulates androgen and RA production, germ cell-mediated, RA-dependent reduction of Leydig cell activity may form a hitherto unknown intratesticular negative feedback loop.
Genes / Markers
Figures
Figure Gallery (8 images)
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Expression
No data available
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
uua2TgTransgenic Insertion
    zf45TgTransgenic Insertion
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      Human Disease / Model
      No data available
      Sequence Targeting Reagents
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      Fish
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      Antibodies
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      Orthology
      No data available
      Engineered Foreign Genes
      Marker Marker Type Name
      EGFPEFGEGFP
      mCherryEFGmCherry
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      Mapping
      No data available
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      Citation
      Crespo, D., Assis, L.H.C., van de Kant, H.J.G., de Waard, S., Safian, D., Lemos, M.S., Bogerd, J., Schulz, R.W. (2019) Endocrine and local signaling interact to regulate spermatogenesis in zebrafish: Follicle-stimulating hormone, retinoic acid and androgens. Development (Cambridge, England). 146(21):.