ZFIN ID: ZDB-PUB-191005-13
Zebrafish embryo as a replacement model for initial biocompatibility studies of biomaterials and drug delivery systems
Rothenbücher, T.S.P., Ledin, J., Gibbs, D., Engqvist, H., Persson, C., Billström, G.H.
Date: 2019
Source: Acta biomaterialia   100: 235-243 (Review)
Registered Authors: Ledin, Johan
Keywords: 3Rs, FET test, fish embryo toxicity, high-throughput, zebrafish
MeSH Terms:
  • Animals
  • Biocompatible Materials/pharmacology*
  • Drug Delivery Systems*
  • Embryo, Nonmammalian/physiology*
  • Models, Animal
  • Toxicity Tests
  • Zebrafish/embryology*
PubMed: 31585201 Full text @ Acta Biomater
The development of new biomaterials and drug delivery systems necessitates animal experimentation to demonstrate biocompatibility and therapeutic efficacy. Reduction and replacement of the requirement to conduct experiment using full-grown animals has been achieved through utilising zebrafish embryos, a promising bridge model between in vitro and in vivo research. In this review, we consider how zebrafish embryos have been utilised to test both the biocompatibility of materials developed to interact with the human body and drug release studies. Furthermore, we outline the advantages and limitations of this model and review legal and ethical issues. We anticipate increasing application of the zebrafish model for biomaterial evaluation in the near future.