PUBLICATION
Heat resilience in embryonic zebrafish revealed by an in vivo stress granule reporter
- Authors
- Wang, R., Zhang, H., Du, J., Xu, J.
- ID
- ZDB-PUB-190929-2
- Date
- 2019
- Source
- Journal of Cell Science 132(20): (Journal)
- Registered Authors
- Du, Jiu Lin, Zhang, Hefei
- Keywords
- Early development, G3BP1, Heat shock, In vivo reporter, Stress granule, Stress resilience, Zebrafish
- MeSH Terms
-
- Animals
- Animals, Genetically Modified/embryology
- Animals, Genetically Modified/genetics
- CRISPR-Cas Systems
- Cytoplasmic Granules*/genetics
- Cytoplasmic Granules*/metabolism
- Heat-Shock Response*
- Mesencephalon/embryology*
- Neurons/metabolism*
- RNA Helicases*/genetics
- RNA Helicases*/metabolism
- Zebrafish*/embryology
- Zebrafish*/genetics
- PubMed
- 31558681 Full text @ J. Cell Sci.
Citation
Wang, R., Zhang, H., Du, J., Xu, J. (2019) Heat resilience in embryonic zebrafish revealed by an in vivo stress granule reporter. Journal of Cell Science. 132(20):.
Abstract
Although the regulation of stress granules has become an intensely studied topic, current investigations of stress granule assembly, disassembly and dynamics are mainly performed in cultured cells. Here we report the establishment of a stress granule reporter to facilitate the real-time study of stress granules in vivo Using CRISPR/Cas9, we fused a green fluorescence protein (GFP) to the endogenous G3BP1 in zebrafish. The GFP-G3BP1 reporter faithfully and robustly responded to heat stress in zebrafish embryos and larvae. The induction of stress granules varied by brain regions under the same stress condition, with the midbrain cells showing the highest efficiency and dynamics. Furthermore, preconditioning using lower heat stress significantly limited stress granule formation during subsequent heat stress. More interestingly, the stress granule formation was much more robust in zebrafish embryos than in larvae and coincided with significant elevated phosphorylated eIF2α and enhanced heat resilience. Therefore, these findings have generated new insights into stress response in zebrafish during early development and demonstrated that the GFP-G3BP1 knockin zebrafish could be a valuable tool for the investigation of stress granule biology.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping