PUBLICATION
Toxic effects of flufenoxuron on development and vascular formation during zebrafish embryogenesis
- Authors
- Park, S., Lee, J.Y., Park, H., Song, G., Lim, W.
- ID
- ZDB-PUB-190928-10
- Date
- 2019
- Source
- Aquatic toxicology (Amsterdam, Netherlands) 216: 105307 (Journal)
- Registered Authors
- Keywords
- Angiogenesis, Development, Embryotoxicity, Flufenoxuron, Zebrafish
- MeSH Terms
-
- Animals
- Embryo, Nonmammalian/blood supply*
- Embryo, Nonmammalian/drug effects
- Embryo, Nonmammalian/metabolism
- Embryonic Development/drug effects*
- Environmental Exposure
- Epithelial Cells/drug effects
- Epithelial Cells/metabolism
- Human Umbilical Vein Endothelial Cells/drug effects
- Humans
- Neovascularization, Physiologic/drug effects*
- Phenylurea Compounds/chemistry
- Phenylurea Compounds/toxicity*
- Toxicity Tests*
- Water Pollutants, Chemical/toxicity
- Zebrafish/embryology*
- Zebrafish/metabolism
- PubMed
- 31557631 Full text @ Aquat. Toxicol.
Citation
Park, S., Lee, J.Y., Park, H., Song, G., Lim, W. (2019) Toxic effects of flufenoxuron on development and vascular formation during zebrafish embryogenesis. Aquatic toxicology (Amsterdam, Netherlands). 216:105307.
Abstract
Flufenoxuron, a chitin synthesis inhibitor that is widely used in developed countries as an insecticide, is rarely degraded in the environment. In addition to that in insects, flufenoxuron-mediated non-targeted death in organisms such as lizards and bees has been reported. However, the toxic effects of this compound on vascular development during embryogenesis, as well as the underlying mechanism, have not yet been elucidated. In the present study, we assessed abnormal development and cardiovascular damage induced by flufenoxuron in zebrafish embryos. Exposed zebrafish had malformed eyes and pathological characteristics such as heart and yolk sac edema. In accordance with developmental inhibition, cell cycle regulatory genes were dysregulated in zebrafish embryos upon exposure to flufenoxuron. We also discovered that this agent can disrupt vascular formation by interfering with angiogenesis-associated genes including the genes encoding vascular endothelial growth factor Aa (vegfaa), vegfc, fms-related tyrosine kinase 1 (flt1), and flt4 in zebrafish embryos. These anti-angiogenic effects of flufenoxuron were further verified using a well-known angiogenesis model, namely human umbilical vein endothelial cells. In conclusion, our results suggest that flufenoxuron inhibits overall development and angiogenesis during embryogenesis.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping