PUBLICATION
Modeling psychiatric comorbid symptoms of epileptic seizures in zebrafish
- Authors
- Canzian, J., Müller, T.E., Franscescon, F., Michelotti, P., Fontana, B.D., Costa, F.V., Rosemberg, D.B.
- ID
- ZDB-PUB-190923-2
- Date
- 2019
- Source
- Journal of psychiatric research 119: 14-22 (Journal)
- Registered Authors
- Keywords
- Anxiety, Epilepsy-related comorbidities, Exploratory activity, Memory deficits, Social behavior, Zebrafish
- MeSH Terms
-
- Animals
- Anxiety/chemically induced
- Anxiety/physiopathology*
- Behavior, Animal/drug effects
- Behavior, Animal/physiology*
- Disease Models, Animal
- Female
- GABA Antagonists/pharmacology
- Humans
- Locomotion/drug effects
- Locomotion/physiology*
- Male
- Memory Disorders/chemically induced
- Memory Disorders/physiopathology*
- Pentylenetetrazole/pharmacology
- Retention, Psychology/drug effects
- Retention, Psychology/physiology
- Seizures/chemically induced
- Seizures/complications
- Seizures/physiopathology*
- Social Behavior*
- Zebrafish
- PubMed
- 31542703 Full text @ J Psychiatr Res
Citation
Canzian, J., Müller, T.E., Franscescon, F., Michelotti, P., Fontana, B.D., Costa, F.V., Rosemberg, D.B. (2019) Modeling psychiatric comorbid symptoms of epileptic seizures in zebrafish. Journal of psychiatric research. 119:14-22.
Abstract
Epilepsy is a debilitating neurological disorder characterized by recurrent unprovoked seizures. Anxiety, cognitive deficits, depressive-like symptoms, and social dysfunction are psychiatric comorbidities with high prevalence in epileptic patients. Due to the genetic and behavioral tractability, the zebrafish is a promising model organism to understand the neural bases involved in epilepsy-related comorbidities. Here, we aimed to characterize some behavioral phenotypes paralleling those observed in epilepsy-related comorbidities after a single pentylenetetrazole (PTZ) exposure in zebrafish. We also analyzed the influence of whole-body cortisol levels in the behavioral responses measured. Fish were exposed to 10 mM PTZ for 20 min to induce epileptic seizures. After 24 h recovery period, locomotion and anxiety-like responses (novel tank and light-dark tests), social interaction (shoaling behavior task), and memory retention (inhibitory avoidance protocol) were assessed. Basically, PTZ impaired habituation to novelty stress, evoked anxiogenic-like behaviors, disrupted shoaling, and caused memory consolidation deficits in zebrafish without changing whole-body cortisol levels. In conclusion, our novel findings further validate the use of zebrafish as a suitable tool for modeling epilepsy-related comorbidities in translational neuropsychiatric research.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping