PUBLICATION
Exposure to humidifier disinfectants induces developmental effects and disrupts thyroid endocrine systems in zebrafish larvae
- Authors
- Kim, H., Ji, K.
- ID
- ZDB-PUB-190921-5
- Date
- 2019
- Source
- Ecotoxicology and environmental safety 184: 109663 (Journal)
- Registered Authors
- Keywords
- Development, HPT axis, Humidifier disinfectants, Oxidative stress, PHMG
- MeSH Terms
-
- Disinfectants/toxicity*
- Humidifiers/standards*
- Larva/drug effects
- Larva/growth & development
- Animals
- Republic of Korea
- Thyroid Gland/drug effects
- Thyroid Gland/growth & development
- Thyroid Hormones/metabolism
- Zebrafish/growth & development*
- Endocrine Disruptors/toxicity*
- PubMed
- 31539807 Full text @ Ecotoxicol. Environ. Saf.
Citation
Kim, H., Ji, K. (2019) Exposure to humidifier disinfectants induces developmental effects and disrupts thyroid endocrine systems in zebrafish larvae. Ecotoxicology and environmental safety. 184:109663.
Abstract
Humidifier disinfectants have been widely used in Korea to prevent the growth of microorganisms in humidifier water. However, their use has been banned since 2011 after epidemiological studies reported humidifier disinfectant induced lung injury. In the present study, the developmental effects of exposure to two humidifier disinfectants (Oxy® and Wiselect) and their main component, polyhexamethylene guanidine (PHMG)-phosphate, were investigated in zebrafish embryos/larvae for seven days. The effects on triiodothyronine (T3) and thyroxine (T4) hormones, reactive oxygen species (ROS) generation, antioxidant enzyme activities, and changes in expression of the genes related to the hypothalamus-pituitary-thyroid (HPT) axis and oxidative stress were also investigated. Zebrafish embryos exposed to the highest concentration (amounts recommended for use by the manufacturers) of all tested humidifier disinfectants showed an increase in embryo coagulation, leading to death without hatching. Exposure to Oxy® and Wiselect resulted in significantly decreased body length, increased ROS generation and antioxidant enzyme activities, decreased T4, and up-regulated genes related to the HPT axis (trh, trβ, and tpo) and oxidative damage (sod2 and gpx1b). The humidifier disinfectants and PHMG-phosphate could induce oxidative stress and disrupt thyroid hormone systems in zebrafish, leading to developmental retardation when used at sub-lethal concentrations. Potential effects of long-term exposure to humidifier disinfectants and mixture effects of several major components deserve further investigation.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping