PUBLICATION
The Neuropeptide Galanin Is Required for Homeostatic Rebound Sleep following Increased Neuronal Activity
- Authors
- Reichert, S., Pavón Arocas, O., Rihel, J.
- ID
- ZDB-PUB-190921-1
- Date
- 2019
- Source
- Neuron 104(2): 370-384.e5 (Journal)
- Registered Authors
- Reichert, Sabine, Rihel, Jason
- Keywords
- galanin, sleep deprivation, sleep homeostasis, sleep pressure, zebrafish
- MeSH Terms
-
- 4-Aminopyridine/pharmacology
- Aconitine/pharmacology
- Animals
- Caffeine/pharmacology
- Extracellular Signal-Regulated MAP Kinases/metabolism
- GABA Antagonists/pharmacology*
- Galanin/drug effects*
- Galanin/genetics
- Galanin/metabolism
- Homeostasis
- Mutation
- Neurons/drug effects*
- Neurons/metabolism
- Pentylenetetrazole/pharmacology*
- Phosphorylation
- Potassium Channel Blockers/pharmacology
- Preoptic Area
- Purinergic P1 Receptor Antagonists/pharmacology
- Sleep/drug effects*
- Sleep/genetics
- Sleep Deprivation/metabolism*
- Voltage-Gated Sodium Channel Agonists/pharmacology
- Wakefulness/drug effects*
- Wakefulness/genetics
- Zebrafish
- PubMed
- 31537465 Full text @ Neuron
Citation
Reichert, S., Pavón Arocas, O., Rihel, J. (2019) The Neuropeptide Galanin Is Required for Homeostatic Rebound Sleep following Increased Neuronal Activity. Neuron. 104(2):370-384.e5.
Abstract
Sleep pressure increases during wake and dissipates during sleep, but the molecules and neurons that measure homeostatic sleep pressure remain poorly understood. We present a pharmacological assay in larval zebrafish that generates short-term increases in wakefulness followed by sustained rebound sleep after washout. The intensity of global neuronal activity during drug-induced wakefulness predicted the amount of subsequent rebound sleep. Whole-brain mapping with the neuronal activity marker phosphorylated extracellular signal-regulated kinase (pERK) identified preoptic Galanin (Galn)-expressing neurons as selectively active during rebound sleep, and the relative induction of galn transcripts was predictive of total rebound sleep time. Galn is required for sleep homeostasis, as galn mutants almost completely lacked rebound sleep following both pharmacologically induced neuronal activity and physical sleep deprivation. These results suggest that Galn plays a key role in responding to sleep pressure signals derived from neuronal activity and functions as an output arm of the vertebrate sleep homeostat.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping