PUBLICATION
Embryonic Exposure to 2,2',3,5',6-pentachlorobiphenyl (PCB-95) Causes Developmental Malformations in Zebrafish
- Authors
- Ranasinghe, P., Thorn, R.J., Seto, R., Creton, R., Bridges, W.C., Chapman, S.C., Lee, C.M.
- ID
- ZDB-PUB-190910-16
- Date
- 2019
- Source
- Environmental toxicology and chemistry 39(1): 162-170 (Journal)
- Registered Authors
- Creton, Robbert
- Keywords
- PCB-95, aquatic toxicology, embryonic development, neurotoxicity, polychlorinated biphenyls, zebrafish
- MeSH Terms
-
- Animals
- Calcium Signaling/drug effects
- Dose-Response Relationship, Drug
- Embryo, Nonmammalian/abnormalities
- Embryo, Nonmammalian/drug effects*
- Embryonic Development/drug effects*
- Models, Theoretical
- Neurons/drug effects
- Neurons/metabolism
- Polychlorinated Biphenyls/toxicity*
- Water Pollutants, Chemical/toxicity*
- Zebrafish/abnormalities*
- PubMed
- 31499578 Full text @ Environ. Toxicol. Chem.
Citation
Ranasinghe, P., Thorn, R.J., Seto, R., Creton, R., Bridges, W.C., Chapman, S.C., Lee, C.M. (2019) Embryonic Exposure to 2,2',3,5',6-pentachlorobiphenyl (PCB-95) Causes Developmental Malformations in Zebrafish. Environmental toxicology and chemistry. 39(1):162-170.
Abstract
2,2',3,5',6-Pentachlorobiphenyl (PCB-95) is an environmental neurotoxicant. There is accumulated evidence that some neurotoxic effects of PCB-95 are caused by increased spontaneous Ca2+ oscillations in neurons by modifying ryanodine receptors (RyR) in calcium releasing channels. However, there are large gaps in explaining brain and other developmental malformations upon embryonic PCB-95 exposure. Here, we addressed those deficiencies by studying the toxic effects of PCB-95 using zebrafish as an ontogenetic model. To characterize these effects, zebrafish embryos with intact chorions were exposed to four different concentrations of PCB-95 (0.25, 0.5, 0.75 and 1ppm) for three consecutive days. The controls were maintained in 0.5X E2 media or egg water, and 0.1% (v/v) of dimethyl sulfoxide (DMSO)/0.5X E2 media or egg water. PCB-95 treated groups showed dose dependent decreases in survival and hatching rates, with increased rates of developmental malformations when compared to controls. These include morphological malformations, brain cell necrosis and smaller eye sizes at five days post fertilization. These data suggest potential mechanisms underlying the abnormal behavior observed in a visual stimulus assay. This study provides insight into PCB-95 induced developmental toxicity and supports the use of the zebrafish model in understanding the effects of PCB-95 exposure. This article is protected by copyright. All rights reserved.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping