PUBLICATION
Cancer-associated fibroblast regulate proliferation and migration of prostate cancer cells through TGF-β signaling pathway
- Authors
- Sun, D.Y., Wu, J.Q., He, Z.H., He, M.F., Sun, H.B.
- ID
- ZDB-PUB-190830-19
- Date
- 2019
- Source
- Life sciences 235: 116791 (Journal)
- Registered Authors
- Keywords
- CAFs, Prostate cancer, TGF-β, Tumor microenvironment, Zebrafish
- MeSH Terms
-
- Animals
- Cancer-Associated Fibroblasts*
- Cell Movement*
- Cell Proliferation*
- Embryo, Nonmammalian/drug effects
- Embryo, Nonmammalian/metabolism
- Embryo, Nonmammalian/pathology*
- Gene Expression Regulation, Neoplastic
- Humans
- Male
- Prostatic Neoplasms/drug therapy
- Prostatic Neoplasms/metabolism
- Prostatic Neoplasms/pathology*
- Pyrazoles/pharmacology*
- Pyrroles/pharmacology*
- Receptors, Transforming Growth Factor beta/antagonists & inhibitors
- Signal Transduction
- Transforming Growth Factor beta1/genetics
- Transforming Growth Factor beta1/metabolism*
- Tumor Cells, Cultured
- Xenograft Model Antitumor Assays
- Zebrafish
- PubMed
- 31465732 Full text @ Life Sci.
Citation
Sun, D.Y., Wu, J.Q., He, Z.H., He, M.F., Sun, H.B. (2019) Cancer-associated fibroblast regulate proliferation and migration of prostate cancer cells through TGF-β signaling pathway. Life sciences. 235:116791.
Abstract
Aims Prostate cancer (PCa) incidence rates are rising in China currently. Cancer-associated fibroblasts (CAFs), as a major component of tumor microenvironment, are crucial for tumor progression. This study was aimed to explore the promotion effect of patient-derived CAFs on the proliferation and migration of prostate cancer cells.
Main methods CAFs were isolated from tumor tissues of PCa patients. The promotion effect of CAFs on the proliferation and migration of PC-3 and LNCaP cells were evaluated in vitro and in vivo. The concentration of TGF-β1 was measured by Luminex assay. The blocking activity of LY2109761 on the promotion effect of CAFs was also evaluated.
Key findings CAFs could significantly promote the proliferation and migration of PC-3 and LNCaP cells both in vitro and in vivo. TGF-β1 was identified as a highly increased factor in CAFs-CM compared with the normal culture medium of these two cancer cell lines. TGF-β receptor inhibitor LY2109761 could suppress the CAFs-induced cellular proliferation and migration of PC-3 cells but not LNCaP cells.
Significance Our study suggested a crucial role for CAFs and TGF-β signaling in the progression of PCa. Zebrafish xenograft model was an ideal animal model for the study of CAFs and cancer cell interaction.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping