PUBLICATION
Environmental chemicals differentially affect epigenetic-related mechanisms in the zebrafish liver (ZF-L) cell line and in zebrafish embryos
- Authors
- Blanc, M., Rüegg, J., Scherbak, N., Keiter, S.H.
- ID
- ZDB-PUB-190826-9
- Date
- 2019
- Source
- Aquatic toxicology (Amsterdam, Netherlands) 215: 105272 (Journal)
- Registered Authors
- Keywords
- Chemical pollutant, DNA methylation, Danio rerio, Histone modification, LUMA, qPCR
- MeSH Terms
-
- Animals
- DNA Methylation/drug effects
- DNA Methylation/genetics
- Embryo, Nonmammalian/drug effects
- Embryo, Nonmammalian/metabolism*
- Epigenesis, Genetic*/drug effects
- Hepatocytes/drug effects
- Hepatocytes/metabolism
- Liver/drug effects
- Liver/metabolism*
- Toxicity Tests, Acute
- Transcription, Genetic/drug effects
- Water Pollutants, Chemical/toxicity*
- Zebrafish/embryology*
- Zebrafish/genetics*
- PubMed
- 31442592 Full text @ Aquat. Toxicol.
- CTD
- 31442592
Citation
Blanc, M., Rüegg, J., Scherbak, N., Keiter, S.H. (2019) Environmental chemicals differentially affect epigenetic-related mechanisms in the zebrafish liver (ZF-L) cell line and in zebrafish embryos. Aquatic toxicology (Amsterdam, Netherlands). 215:105272.
Abstract
A number of chemicals have been shown to affect epigenetic patterning and functions. Since epigenetic mechanisms regulate transcriptional networks, epigenetic changes induced by chemical exposure can represent early molecular events for long-term adverse physiological effects. Epigenetics has thus appeared as a research field of major interest within (eco)toxicological sciences. The present study aimed at measuring effects on epigenetic-related mechanisms of selected environmental chemicals (bisphenols, perfluorinated chemicals, methoxychlor, permethrin, vinclozolin and coumarin 47) in zebrafish embryos and liver cells (ZFL). Transcription of genes related to DNA methylation and histone modifications was measured and global DNA methylation was assessed in ZFL cells using the LUMA assay. The differences in results gathered from both models suggest that chemicals affect different mechanisms related to epigenetics in embryos and cells. In zebrafish embryos, exposure to bisphenol A, coumarin 47, methoxychlor and permethrin lead to significant transcriptional changes in epigenetic factors suggesting that they can impact early epigenome reprogramming related to embryonic development. In ZFL cells, significant transcriptional changes were observed upon exposure to all chemicals but coumarin 47; however, only perfluorooctane sulfonate induced significant effects on global DNA methylation. Notably, in contrast to the other tested chemicals, perfluorooctane sulfonate affected only the expression of the histone demethylase kdm5ba. In addition, kdm5ba appeared as a sensitive gene in zebrafish embryos as well. Taken together, the present results suggest a role for kdm5ba in regulating epigenetic patterns in response to chemical exposure, even though mechanisms remain unclear. To confirm these findings, further evidence is required regarding changes in site-specific histone marks and DNA methylation together with their long-term effects on physiological outcomes.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping