PUBLICATION
Transcriptional autoregulation of zebrafish tbx6 is required for somite segmentation
- Authors
- Ban, H., Yokota, D., Otosaka, S., Kikuchi, M., Kinoshita, H., Fujino, Y., Yabe, T., Ovara, H., Izuka, A., Akama, K., Yamasu, K., Takada, S., Kawamura, A.
- ID
- ZDB-PUB-190826-12
- Date
- 2019
- Source
- Development (Cambridge, England) 146(18): (Journal)
- Registered Authors
- Kawamura, Akinori, Takada, Shinji, Yamasu, Kyo
- Keywords
- Enhancer, Somite segmentation, T-box transcription factor, Transcriptional regulation, Zebrafish
- MeSH Terms
-
- Animals
- Binding Sites/genetics
- Body Patterning/genetics*
- Embryo, Nonmammalian/metabolism
- Enhancer Elements, Genetic/genetics
- Gene Deletion
- Gene Expression Regulation, Developmental
- Genes, Reporter
- Homeostasis/genetics*
- Homozygote
- Protein Domains
- RNA, Messenger/genetics
- RNA, Messenger/metabolism
- Somites/embryology*
- Somites/metabolism
- T-Box Domain Proteins/chemistry
- T-Box Domain Proteins/genetics
- T-Box Domain Proteins/metabolism*
- Transcription, Genetic*
- Zebrafish/embryology*
- Zebrafish/genetics*
- Zebrafish Proteins/chemistry
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- PubMed
- 31444219 Full text @ Development
Citation
Ban, H., Yokota, D., Otosaka, S., Kikuchi, M., Kinoshita, H., Fujino, Y., Yabe, T., Ovara, H., Izuka, A., Akama, K., Yamasu, K., Takada, S., Kawamura, A. (2019) Transcriptional autoregulation of zebrafish tbx6 is required for somite segmentation. Development (Cambridge, England). 146(18):.
Abstract
The presumptive somite boundary in the presomitic mesoderm (PSM) is defined by the anterior border of the expression domain of Tbx6 protein. During somite segmentation, the expression domain of Tbx6 is regressed by Ripply-meditated degradation of Tbx6 protein. Although the expression of zebrafish tbx6 remains restricted to the PSM, the transcriptional regulation of tbx6 remains poorly understood. Here, we show that the expression of zebrafish tbx6 is maintained by transcriptional autoregulation. We find that a proximal-located cis-regulatory module, TR1, which contains two putative T-box sites, is required for somite segmentation in the intermediate body and for proper expression of segmentation genes. Embryos with deletion of TR1 exhibit significant reduction of tbx6 expression at the 12-somite stage, although its expression is initially observed. Additionally, Tbx6 is associated with TR1 and activates its own expression in the anterior PSM. Furthermore, the anterior expansion of tbx6 expression in ripply mutants is suppressed in a TR1-dependent manner. The results suggest that the autoregulatory loop of zebrafish tbx6 facilitates immediate removal of Tbx6 protein through termination of its own transcription at the anterior PSM.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping