PUBLICATION

Maternal ethanol consumption before paternal fertilization: Stimulation of hypocretin neurogenesis and ethanol intake in zebrafish offspring

Authors
Collier, A.D., Min, S.S., Campbell, S.D., Roberts, M.Y., Camidge, K., Leibowitz, S.F.
ID
ZDB-PUB-190809-9
Date
2019
Source
Progress in neuro-psychopharmacology & biological psychiatry   96: 109728 (Journal)
Registered Authors
Keywords
Ethanol, Hypocretin, Orexin, Preconception alcohol, Zebrafish
MeSH Terms
  • Alcohol Drinking/adverse effects
  • Alcohol Drinking/trends*
  • Animals
  • Animals, Genetically Modified
  • Ethanol/administration & dosage*
  • Ethanol/adverse effects
  • Female
  • Fertilization/drug effects
  • Fertilization/physiology*
  • Locomotion/drug effects
  • Locomotion/physiology
  • Male
  • Neurogenesis/drug effects
  • Neurogenesis/physiology*
  • Orexins/metabolism*
  • Pregnancy
  • Prenatal Exposure Delayed Effects/chemically induced
  • Prenatal Exposure Delayed Effects/metabolism*
  • Zebrafish
PubMed
31394141 Full text @ Prog. Neuropsychopharmacol. Biol. Psychiatry
Abstract
There are numerous clinical and pre-clinical studies showing that exposure of the embryo to ethanol markedly affects neuronal development and stimulates alcohol drinking and related behaviors. In rodents and zebrafish, our studies show that embryonic exposure to low-dose ethanol, in addition increasing voluntary ethanol intake during adolescence, increases the density of hypothalamic hypocretin (hcrt) neurons, a neuropeptide known to regulate reward-related behaviors. The question addressed here in zebrafish is whether maternal ethanol intake before conception also affects neuronal and behavioral development, phenomena suggested by clinical reports but seldom investigated. To determine if preconception maternal ethanol consumption also affects these hcrt neurons and behavior in the offspring, we first standardized a method of measuring voluntary ethanol consumption in AB strain adult and larval zebrafish given gelatin meals containing 10% or 0.1% ethanol, respectively. We found the number of bites of gelatin to be an accurate measure of intake in adults and a strong predictor of blood ethanol levels, and also to be a reliable indicator of intake in larval zebrafish. We then used this feeding paradigm and live imaging to examine the effects of preconception maternal intake of 10% ethanol-gelatin compared to plain-gelatin for 14 days on neuronal development in the offspring. Whereas ethanol consumption by adult female HuC:GFP transgenic zebrafish had no impact on the number of differentiated HuC+ neurons at 28 h post-fertilization (hpf), ethanol consumption by adult female hcrt:EGFP zebrafish significantly increased the number of hcrt neurons in the offspring, an effect observed at 28 hpf and confirmed at 6 and 12 days post-fertilization (dpf). This increase in hcrt neurons was primarily present on the left side of the brain, indicating asymmetry in ethanol's actions, and it was accompanied by behavioral changes in the offspring, including a significant increase in novelty-induced locomotor activity but not thigmotaxis measured at 6 dpf and also in voluntary consumption of 0.1% ethanol-gelatin at 12 dpf. Notably, these measures of ethanol intake and locomotor activity stimulated by preconception ethanol were strongly, positively correlated with the number of hcrt neurons. These findings demonstrate that preconception maternal ethanol consumption affects the brain and behavior of the offspring, producing effects similar to those caused by embryonic ethanol exposure, and they provide further evidence that the ethanol-induced increase in hcrt neurogenesis contributes to the behavioral disturbances caused by ethanol.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping