PUBLICATION
Chlorpyrifos exposure induces lipid metabolism disorder at the physiological and transcriptomic levels in larval zebrafish
- Authors
- Wang, X., Zhou, J., Shen, M., Shen, J., Zhang, X., Jin, Y.
- ID
- ZDB-PUB-190807-17
- Date
- 2019
- Source
- Acta biochimica et biophysica Sinica 51(9): 890-899 (Journal)
- Registered Authors
- Wang, Xiaoyu
- Keywords
- cardiovascular toxicity, chlorpyrifos, larval zebrafish, lipid metabolism, transcriptome analysis
- MeSH Terms
-
- Animals
- Chlorpyrifos/toxicity*
- Disease Models, Animal
- Insecticides/toxicity*
- Larva/drug effects
- Larva/metabolism
- Lipid Metabolism/drug effects*
- Lipid Metabolism Disorders/chemically induced*
- Transcriptome
- Zebrafish*/genetics
- Zebrafish*/metabolism
- PubMed
- 31384916 Full text @ Acta. Biochim. Biophys. Sin (Shanghai)
- CTD
- 31384916
Citation
Wang, X., Zhou, J., Shen, M., Shen, J., Zhang, X., Jin, Y. (2019) Chlorpyrifos exposure induces lipid metabolism disorder at the physiological and transcriptomic levels in larval zebrafish. Acta biochimica et biophysica Sinica. 51(9):890-899.
Abstract
Chlorpyrifos (CPF) is a widely used insecticide in pest control, and it can affect aquatic animals by contaminating the water. In this study, larval zebrafish were exposed to CPF at concentrations of 30, 100 and 300 μg/l for 7 days. In the CPF-treated group, lipid droplet accumulation was reduced in larval zebrafish. The levels of triglyceride (TG), total cholesterol (TC), and pyruvate were also decreased after CPF exposure. Cellular apoptosis were significantly increased in the heart tissue after CPF exposure compared with the control. Transcription changes in cardiovascular genes were also observed. Through transcriptome analysis, we found that the transcription of 465 genes changed significantly, with 398 upregulated and 67 downregulated in the CPF-treated group, indicating that CPF exposure altered the transcription of genes. Among these altered genes, a number of genes were closely related to the glucose and lipid metabolism pathways. Furthermore, we also confirmed that the transcription of genes related to fatty acid synthesis, TC synthesis, and lipogenesis were significantly decreased in larval zebrafish after exposure to CPF. These results indicated that CPF exposure induced lipid metabolism disorders associated with cardiovascular toxicity in larval zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping