PUBLICATION
Effect of low sperm quality on progeny: a study on zebrafish as model species
- Authors
- Riesco, M.F., Valcarce, D.G., Martínez-Vázquez, J.M., Robles, V.
- ID
- ZDB-PUB-190803-7
- Date
- 2019
- Source
- Scientific Reports 9: 11192 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Animals
- Biomarkers/analysis
- Biomarkers/metabolism
- Congenital Abnormalities/genetics*
- Disease Models, Animal
- Embryo, Nonmammalian
- Female
- Fertilization/genetics*
- Fertilization in Vitro/adverse effects
- Fertilization in Vitro/standards*
- Gene Expression Regulation, Developmental
- Humans
- Infertility/therapy
- Male
- MicroRNAs/analysis
- MicroRNAs/metabolism*
- Semen Analysis/methods
- Semen Analysis/standards
- Sperm Motility/genetics
- Spermatozoa/metabolism*
- Up-Regulation
- Zebrafish
- Zebrafish Proteins/genetics
- PubMed
- 31371755 Full text @ Sci. Rep.
Citation
Riesco, M.F., Valcarce, D.G., Martínez-Vázquez, J.M., Robles, V. (2019) Effect of low sperm quality on progeny: a study on zebrafish as model species. Scientific Reports. 9:11192.
Abstract
Nowadays a decrease tendency in human sperm quality has been reported mainly in developed countries. Reproductive technologies have been very valuable in achieving successful pregnancies with low quality sperm samples. However, considering that spermatozoa molecular contribution is increasingly important in recent studies, it is crucial to study whether fertilization with low sperm quality could leave a molecular mark on progeny. This study explores the consequences that fertilization with low sperm quality may have on progeny, using zebrafish as a model. Good and bad breeders were established attending to sperm quality analyses and were individually tracked. Significant differences in fertilization and malformation rates were obtained in progenies between high and low quality sperm samples. Moreover an altered miR profile was found in the progenies of bad zebrafish breeders (upregulation of miR-141 and miR -122 in 24 hpf embryos) and as a consequence, some of their targets involved in male sex development such as dmrt1, suffered downregulation. Our results indicate that fertilizing with high sperm quality samples becomes relevant from a new perspective: to avoid molecular alterations in the progeny that could remain masked and therefore produce unexpected consequences in it.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping