PUBLICATION
Histological, biochemical and transcriptomic analyses reveal liver damage in zebrafish (Danio rerio) exposed to phenanthrene
- Authors
- Mai, Y., Peng, S., Li, H., Lai, Z.
- ID
- ZDB-PUB-190803-13
- Date
- 2019
- Source
- Comparative biochemistry and physiology. Toxicology & pharmacology : CBP 225: 108582 (Journal)
- Registered Authors
- Keywords
- Liver, Phenanthrene, Transcriptomics, Zebrafish
- MeSH Terms
-
- Biomarkers/metabolism
- Zebrafish/metabolism*
- Transcriptome*
- Water Pollutants, Chemical/toxicity*
- Phenanthrenes/toxicity*
- Animals
- Glutathione Transferase/metabolism
- Superoxide Dismutase/metabolism
- Oxidative Stress
- Liver*/metabolism
- Liver*/pathology
- Gene Expression Profiling
- PubMed
- 31374294 Full text @ Comp. Biochem. Physiol. C Toxicol. Pharmacol.
Citation
Mai, Y., Peng, S., Li, H., Lai, Z. (2019) Histological, biochemical and transcriptomic analyses reveal liver damage in zebrafish (Danio rerio) exposed to phenanthrene. Comparative biochemistry and physiology. Toxicology & pharmacology : CBP. 225:108582.
Abstract
Phenanthrene (PHE) is a common polycyclic aromatic hydrocarbon (PAH) in aquatic environments, and this contaminant can cause adverse effects on teleostean performance. In this study, we exposed the model freshwater fish (zebrafish; Danio rerio) to 300 μg/L PHE for 15 days. Histological analysis demonstrated that liver morphology deteriorated in PHE-exposed zebrafish, and cellular damage in the liver increased. Biological analysis revealed that exposure to PHE elicited significant changes in glutathione S-transferases (GST) and superoxide dismutase (SOD) activities. 476 differentially expressed genes (DEGs) were identified in liver between control and PHE treated groups through the transcriptomic analysis. Gene Ontology enrichment analysis (GO) suggested that PHE exposure induced changes in the expression of genes associated with "lipid transporter activity", "catalytic activity", "metal ion binding", "lipid transport" and "transmembrane transport". Furthermore, the "vitamin digestion and absorption" and "fat digestion and absorption" pathways enriched in Kyoto Encyclopedia of Genes and Genomes analysis (KEGG). Additionally, five candidate biomarkers associated with the PHE response in zebrafish were identified. In conclusion, our results elucidate the physiological and molecular responses to PHE exposure in the liver of zebrafish, and provide a framework for further studies of the mechanisms underlying the toxic effects of polycyclic aromatic hydrocarbons (PAHs) on aquatic organisms.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping