PUBLICATION
Ability of prebiotic polysaccharides to activate a HIF1α-antimicrobial peptide axis determines liver injury risk in zebrafish
- Authors
- Zhang, Z., Ran, C., Ding, Q.W., Liu, H.L., Xie, M.X., Yang, Y.L., Xie, Y.D., Gao, C.C., Zhang, H.L., Zhou, Z.G.
- ID
- ZDB-PUB-190803-10
- Date
- 2019
- Source
- Communications biology 2: 274 (Journal)
- Registered Authors
- Ding, Qianwen, Gao, Chenchen, Ran, Chao, Xie, Yadong, Yang, Yalin, Zhang, Zhen, Zhou, Zhigang
- Keywords
- Antimicrobials, Dysbiosis, Microbiota, Non-alcoholic fatty liver disease
- MeSH Terms
-
- Animals
- Antimicrobial Cationic Peptides/biosynthesis
- Antimicrobial Cationic Peptides/metabolism*
- Diet, High-Fat
- Fatty Liver/etiology*
- Gastrointestinal Microbiome
- Hypoxia-Inducible Factor 1, alpha Subunit/metabolism*
- Lactobacillus
- Larva
- Polysaccharides/administration & dosage*
- Prebiotics/administration & dosage*
- Zebrafish/growth & development
- PubMed
- 31372513 Full text @ Commun Biol
Citation
Zhang, Z., Ran, C., Ding, Q.W., Liu, H.L., Xie, M.X., Yang, Y.L., Xie, Y.D., Gao, C.C., Zhang, H.L., Zhou, Z.G. (2019) Ability of prebiotic polysaccharides to activate a HIF1α-antimicrobial peptide axis determines liver injury risk in zebrafish. Communications biology. 2:274.
Abstract
Natural polysaccharides have received much attention for their ability to ameliorate hepatic steatosis induced by high-fat diet. However, the potential risks of their use have been less investigated. Here, we show that the exopolysaccharides (EPS) from Lactobacillus rhamnosus GG (LGG) and L. casei BL23 reduce hepatic steatosis in zebrafish fed a high-fat diet, while BL23 EPS, but not LGG EPS, induce liver inflammation and injury. This is due to the fact that BL23 EPS induces gut microbial dysbiosis, while LGG EPS promotes microbial homeostasis. We find that LGG EPS, but not BL23 EPS, can directly activate intestinal HIF1α, and increased HIF1α boosts local antimicrobial peptide expression to facilitate microbial homeostasis, explaining the distinct compositions of LGG EPS- and BL23 EPS-associated microbiota. Finally, we find that liver injury risk is not confined to Lactobacillus-derived EPS but extends to other types of commonly used natural polysaccharides, depending on their HIF1α activation efficiency.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping