PUBLICATION
The LipoGlo reporter system for sensitive and specific monitoring of atherogenic lipoproteins
- Authors
- Thierer, J.H., Ekker, S.C., Farber, S.A.
- ID
- ZDB-PUB-190802-3
- Date
- 2019
- Source
- Nature communications 10: 3426 (Journal)
- Registered Authors
- Ekker, Stephen C., Farber, Steven
- Keywords
- none
- MeSH Terms
-
- Animals
- Apolipoproteins B/chemistry*
- Apolipoproteins B/metabolism
- Atherosclerosis/pathology*
- Humans
- Larva/metabolism
- Lipoproteins, LDL/analysis*
- Luciferases/chemistry*
- Luciferases/metabolism
- Staining and Labeling/methods*
- Zebrafish/embryology
- PubMed
- 31366908 Full text @ Nat. Commun.
Citation
Thierer, J.H., Ekker, S.C., Farber, S.A. (2019) The LipoGlo reporter system for sensitive and specific monitoring of atherogenic lipoproteins. Nature communications. 10:3426.
Abstract
Apolipoprotein-B (ApoB) is the structural component of atherogenic lipoproteins, lipid-rich particles that drive atherosclerosis by accumulating in the vascular wall. As atherosclerotic cardiovascular disease is the leading cause of death worldwide, there is an urgent need to develop new strategies to prevent lipoproteins from causing vascular damage. Here we report the LipoGlo system, which uses a luciferase enzyme (NanoLuc) fused to ApoB to monitor several key determinants of lipoprotein atherogenicity including particle abundance, size, and localization. Using LipoGlo, we comprehensively characterize the lipoprotein profile of individual larval zebrafish and collect images of atherogenic lipoprotein localization in an intact organism. We report multiple extravascular lipoprotein localization patterns, as well as identify Pla2g12b as a potent regulator of lipoprotein size. ApoB-fusion proteins thus represent a sensitive and specific approach to study atherogenic lipoproteins and their genetic and small molecule modifiers.
Errata / Notes
This article is corrected by ZDB-PUB-220906-209 .
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping