PUBLICATION
Bioconcentration, depuration and toxicity of Pb in the presence of titanium dioxide nanoparticles in zebrafish larvae
- Authors
- Hu, S., Han, J., Yang, L., Li, S., Guo, Y., Zhou, B., Wu, H.
- ID
- ZDB-PUB-190724-39
- Date
- 2019
- Source
- Aquatic toxicology (Amsterdam, Netherlands) 214: 105257 (Journal)
- Registered Authors
- Guo, YongYong, Yang, LiHua, Zhou, BingSheng
- Keywords
- Bioconcentration, Depuration, Lead, Titanium dioxide nanoparticles, Toxicity, Zebrafish larvae
- MeSH Terms
-
- Adsorption
- Animals
- Behavior, Animal/drug effects
- Biological Availability
- Larva/drug effects
- Larva/genetics
- Larva/ultrastructure
- Lead/toxicity*
- Locomotion/drug effects
- Metallothionein/metabolism
- Nanoparticles/toxicity*
- Nanoparticles/ultrastructure
- Suspensions
- Titanium/toxicity*
- Transcription, Genetic/drug effects
- Water Pollutants, Chemical/toxicity
- Zebrafish/genetics
- Zebrafish/metabolism*
- PubMed
- 31336221 Full text @ Aquat. Toxicol.
Citation
Hu, S., Han, J., Yang, L., Li, S., Guo, Y., Zhou, B., Wu, H. (2019) Bioconcentration, depuration and toxicity of Pb in the presence of titanium dioxide nanoparticles in zebrafish larvae. Aquatic toxicology (Amsterdam, Netherlands). 214:105257.
Abstract
The interactions between nanoparticles (NPs) and metals in aquatic environments may modify the bioavailability and toxicity of metals to organisms. In this study, we investigated the effects of titanium dioxide NPs (n-TiO2) on the bioconcentration, depuration, and neurotoxic effects of lead (Pb) in zebrafish larvae. Transmission electron microscopy coupled with energy-dispersive X-ray spectroscopy showed that Pb2+ was adsorbed by n-TiO2 to form NP-Pb complexes in suspension, and these complexes were observed in larval tissues. The bioconcentration of Pb in larvae along with the depuration rates of Pb were higher in the presence of n-TiO2 compared to when n-TiO2 was absent. Exposure to Pb alone induced the expression of the biomarker metallothionein, downregulated neurodevelopment-related genes, and reduced swimming activity of larvae. However, the addition of n-TiO2 to the exposure solution alleviated these effects. The results suggest that n-TiO2 can act as a carrier of Pb to increase its bioconcentration; however, the formation of NP-Pb complexes likely reduces the amount of free Pb2+, thereby reducing toxicity to larvae.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping