PUBLICATION
Embryonic exposure to soil samples from a gangue stacking area induces thyroid hormone disruption in zebrafish
- Authors
- Yang, F., Li, G., Sang, N.
- ID
- ZDB-PUB-190724-29
- Date
- 2019
- Source
- Chemosphere 236: 124337 (Journal)
- Registered Authors
- Keywords
- Coal gangue stacking, Developmental toxicity, Hypothalamus-pituitary-thyroid axis, Village soil sample, Zebrafish embryo
- MeSH Terms
-
- Animals
- Coal Industry/trends*
- Soil/chemistry*
- Thyroid Hormones/adverse effects*
- Thyroid Hormones/metabolism*
- Zebrafish/embryology*
- PubMed
- 31330433 Full text @ Chemosphere
Citation
Yang, F., Li, G., Sang, N. (2019) Embryonic exposure to soil samples from a gangue stacking area induces thyroid hormone disruption in zebrafish. Chemosphere. 236:124337.
Abstract
The total accumulative stockpiles of gangue from long-term coal mining exceed 1 billion tons and occupy 182 square kilometers, and 50 million tons of additional gangue are generated per year in Shanxi, a major energy province in China. The objective of this study was to examine whether exposure to village soils affected by gangue stacking would disrupt thyroid hormone system homeostasis and eventually affect endocrine system and development, using zebrafish (Danio rerio) as a model organism. The zebrafish embryos were exposed to village soil leachates at 0, 1:9, 1:3 and 1:1 from 1 to 120 h postfertilization (hpf), and the sample caused a dose-dependent increase in the mortality and malformation rate, and decrease in the heart rate, hatching rate and body length of zebrafish larvae. Importantly, the soil leachate alleviated the whole-body triiodothyronine (T3) and thyroxine (T4) levels at higher concentrations, and altered the expression of the hypothalamic-pituitary-thyroid (HPT) axis-regulating genes crh, trh, tshβ, nis, tg, nkx2.1, pax8, hhex, ttr, dio1, dio2, ugt1ab, trα, and trβ and the PAH exposure-related genes ahr2 and cyp1a. These findings highlight the potential risk of thyroid hormone disruption and developmental toxicity from soil samples around coal gangue stacking areas.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping