PUBLICATION
The size and composition of polymeric nanocapsules dictate their interaction with macrophages and biodistribution in zebrafish
- Authors
- Crecente-Campo, J., Guerra-Varela, J., Peleteiro, M., Gutiérrez-Lovera, C., Fernández-Mariño, I., Diéguez-Docampo, A., González-Fernández, Á., Sánchez, L., Alonso, M.J.
- ID
- ZDB-PUB-190716-9
- Date
- 2019
- Source
- Journal of controlled release : official journal of the Controlled Release Society 308: 98-108 (Journal)
- Registered Authors
- Keywords
- Chitosan, Inulin, Macrophages, Nanocapsules, Particle size, Zebrafish
- MeSH Terms
-
- Animals
- Chitosan/chemistry*
- Inulin/chemistry*
- Macrophages/metabolism*
- Mice
- Nanocapsules*
- Particle Size
- Polymers/chemistry
- RAW 264.7 Cells
- Tissue Distribution
- Zebrafish
- PubMed
- 31306677 Full text @ J. Control Release
Citation
Crecente-Campo, J., Guerra-Varela, J., Peleteiro, M., Gutiérrez-Lovera, C., Fernández-Mariño, I., Diéguez-Docampo, A., González-Fernández, Á., Sánchez, L., Alonso, M.J. (2019) The size and composition of polymeric nanocapsules dictate their interaction with macrophages and biodistribution in zebrafish. Journal of controlled release : official journal of the Controlled Release Society. 308:98-108.
Abstract
Macrophages are pivotal cells of the innate immune system specialized in the phagocytosis of foreign elements. Nanoparticles intentionally designed to target macrophages and modulate their response are of especial interest in the case of chronic inflammatory diseases, cancer and for vaccine development. This work aimed to understand the role of size and shell composition of polymeric nanocapsules (NCs) in their interaction with macrophages, both in vitro and in vivo. A systematic study was performed using two different sizes of inulin and chitosan NCs, negatively and positively charged, respectively, small (≈ 70 nm) and medium (170-250 nm). The in vitro results showed that small NCs interacted more efficiently with macrophages than their larger counterparts. Inulin NCs were significantly less toxic than chitosan NCs. Finally, following in vivo administration (intravenous/intramuscular) to zebrafish, small NCs, regardless of their composition, disseminated considerably faster and further than their medium size counterparts. These results emphasize how small changes in the nanometric range can lead to a remarkably different interaction with the immune cells and biodistribution profile.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping