PUBLICATION
Pharmacological modulation of melanocortin-4 receptor by melanocortin receptor accessory protein 2 in Nile tilapia
- Authors
- Wang, M., Chen, Y., Zhu, M., Xu, B., Guo, W., Lyu, Y., Zhang, C.
- ID
- ZDB-PUB-190713-7
- Date
- 2019
- Source
- General and comparative endocrinology 282: 113219 (Journal)
- Registered Authors
- Keywords
- Melanocortin 2 receptor accessory protein 2, Melanocortin-4 receptor, Tilapia
- MeSH Terms
-
- Adaptor Proteins, Signal Transducing/metabolism*
- Amino Acid Sequence
- Animals
- Cell Membrane/metabolism
- Cichlids/metabolism*
- Conserved Sequence
- Evolution, Molecular
- HEK293 Cells
- Humans
- Phylogeny
- Protein Binding
- Receptor, Melanocortin, Type 4/chemistry
- Receptor, Melanocortin, Type 4/genetics
- Receptor, Melanocortin, Type 4/metabolism*
- Signal Transduction
- Synteny/genetics
- Tissue Distribution
- PubMed
- 31299224 Full text @ Gen. Comp. Endocrinol.
Citation
Wang, M., Chen, Y., Zhu, M., Xu, B., Guo, W., Lyu, Y., Zhang, C. (2019) Pharmacological modulation of melanocortin-4 receptor by melanocortin receptor accessory protein 2 in Nile tilapia. General and comparative endocrinology. 282:113219.
Abstract
The melanocortin-4 receptor (MC4R) acts as a member of G-protein coupled receptors and participate in food intake and energy expenditure. Melanocortin 2 receptor accessory protein 2 (MRAP2) plays a critical role in regulating MC4R signaling in mammals and zebrafish. However, evidence on their interaction in other teleost species remains elusive. Here, we cloned and assessed the evolutionary aspect and pharmacological modulation of MRAP2 on MC4R signaling in Nile tilapia (Oreochromis niloticus). Tissue distribution analysis of tmc4r and tmrap2 confirmed their co-expression in the brain region. tMRAP2 protein could form antiparallel homo-dimer and directly interacted with tMC4R in vitro and presence of tMRAP2 led to the reduction of agonist response and surface expression of tMC4R. Overall, our findings provide a comparative overview on the evolutionary conservation, genomic distribution, tissue-specific expression and pharmacological profile of the MC4R and MRAP2 in another non-mammalian teleost.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping