PUBLICATION
Diffraction-limited axial scanning in thick biological tissue with an aberration-correcting adaptive lens
- Authors
- Philipp, K., Lemke, F., Scholz, S., Wallrabe, U., Wapler, M.C., Koukourakis, N., Czarske, J.W.
- ID
- ZDB-PUB-190704-3
- Date
- 2019
- Source
- Scientific Reports 9: 9532 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
- none
- PubMed
- 31267005 Full text @ Sci. Rep.
Citation
Philipp, K., Lemke, F., Scholz, S., Wallrabe, U., Wapler, M.C., Koukourakis, N., Czarske, J.W. (2019) Diffraction-limited axial scanning in thick biological tissue with an aberration-correcting adaptive lens. Scientific Reports. 9:9532.
Abstract
Diffraction-limited deep focusing into biological tissue is challenging due to aberrations that lead to a broadening of the focal spot. The diffraction limit can be restored by employing aberration correction for example with a deformable mirror. However, this results in a bulky setup due to the required beam folding. We propose a bi-actuator adaptive lens that simultaneously enables axial scanning and the correction of specimen-induced spherical aberrations with a compact setup. Using the bi-actuator lens in a confocal microscope, we show diffraction-limited axial scanning up to 340 μm deep inside a phantom specimen. The application of this technique to in vivo measurements of zebrafish embryos with reporter-gene-driven fluorescence in a thyroid gland reveals substructures of the thyroid follicles, indicating that the bi-actuator adaptive lens is a meaningful supplement to the existing adaptive optics toolset.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
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