PUBLICATION
Chronic exposure to environmental concentrations of phenanthrene impairs zebrafish reproduction
- Authors
- Peng, X., Sun, X., Yu, M., Fu, W., Chen, H., Chen, J.
- ID
- ZDB-PUB-190701-23
- Date
- 2019
- Source
- Ecotoxicology and environmental safety 182: 109376 (Journal)
- Registered Authors
- Keywords
- Endocrine disruption, Phenanthrene (PHE), Reproduction, Sex hormones, Zebrafish
- MeSH Terms
-
- Animals
- Embryo, Nonmammalian/drug effects*
- Embryonic Development/drug effects*
- Estradiol/blood
- Female
- Gonadotropin-Releasing Hormone/blood
- Male
- Phenanthrenes/toxicity*
- Reproduction/drug effects
- Sex Factors
- Testosterone/blood
- Vitellogenins/blood
- Water Pollutants, Chemical/toxicity*
- Zebrafish/blood
- Zebrafish/genetics
- Zebrafish/growth & development*
- PubMed
- 31254851 Full text @ Ecotoxicol. Environ. Saf.
Citation
Peng, X., Sun, X., Yu, M., Fu, W., Chen, H., Chen, J. (2019) Chronic exposure to environmental concentrations of phenanthrene impairs zebrafish reproduction. Ecotoxicology and environmental safety. 182:109376.
Abstract
Phenanthrene (PHE) is a tricyclic polycyclic aromatic hydrocarbon which distributed extensively in the aquatic environment. However, the knowledge about its impact on fish reproduction is still limited, particularly under a chronic exposure regime. In this study, we exposed zebrafish (Danio rerio) embryos to environmentally relevant concentrations (0.2, 1.0, and 5.0 μg/L) of PHE for 4 months and assessed the impact on reproduction. The results demonstrated that egg production was decreased in fish exposed to PHE, with a significant reduction at 5.0 μg/L. The exposure significantly decreased the circulating concentrations of estradiol (E2) and testosterone (T) in female fish or E2 in male fish. In addition, plasma vitellogenin levels were significantly inhibited after PHE exposure in female fish. The transcription of hypothalamic-pituitary-gonadal (HPG) axis related genes (GnRH2, FSHβ, LHβ, 17β-HSD, CYP11A1, and CYP19a) were significantly altered in a sex-specific manner. In addition, embryos derived from exposed parents exhibited increased malformation and decreased hatching success in the F1 generation. Taken together, these results demonstrate that chronic exposure to environmentally relevant concentration of PHE could cause adverse effects on reproduction and impair the development of offspring, ultimately leading to fish population decline in aquatic environment.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping