PUBLICATION
Safety assessment of antibiotic administration by magnetic nanoparticles in in vitro zebrafish liver and intestine cultures
- Authors
- Chemello, G., Randazzo, B., Zarantoniello, M., Fifi, A.P., Aversa, S., Ballarin, C., Radaelli, G., Magro, M., Olivotto, I.
- ID
- ZDB-PUB-190701-21
- Date
- 2019
- Source
- Comparative biochemistry and physiology. Toxicology & pharmacology : CBP 224: 108559 (Journal)
- Registered Authors
- Keywords
- In vitro, Nanoparticles, Oxytetracycline, Tissue culture, Zebrafish
- MeSH Terms
-
- Oxytetracycline/administration & dosage
- Oxytetracycline/toxicity*
- Intestines/drug effects*
- Liver/drug effects*
- Zebrafish
- Anti-Bacterial Agents/administration & dosage
- Anti-Bacterial Agents/toxicity*
- Animals
- Models, Animal
- Magnetite Nanoparticles/administration & dosage*
- Tissue Culture Techniques
- PubMed
- 31254662 Full text @ Comp. Biochem. Physiol. C Toxicol. Pharmacol.
Citation
Chemello, G., Randazzo, B., Zarantoniello, M., Fifi, A.P., Aversa, S., Ballarin, C., Radaelli, G., Magro, M., Olivotto, I. (2019) Safety assessment of antibiotic administration by magnetic nanoparticles in in vitro zebrafish liver and intestine cultures. Comparative biochemistry and physiology. Toxicology & pharmacology : CBP. 224:108559.
Abstract
Different in vitro models have been suggested to replace in vivo studies. In vitro studies are of great interest and give the opportunity to analyze cellular responses in a closed system with stable experimental conditions and to avoid direct animal exposure and distress during the experiments. These methods are useful to test drugs and chemicals toxicity in order to better understand their environmental impact. In the present study, fish organ cultures have been used to test different oxytetracycline exposure methods, including oxide nanoparticles (IONPs), using zebrafish as experimental model. Results showed that oxytetracycline accumulation at the end of the experiment (24 h) in the exposed organs did not show any significant difference in the analyzed samples and was not dependent on the exposure way (free or IONPs-bound oxytetracycline). However, as regards molecular analysis, the different exposure ways tested in this study showed some differences in the expression of genes involved in stress response. The present data did not completely agree with a previous in vivo study performed in zebrafish using IONPs, underlying that replacement of in vivo models with in vitro studies cannot always represent the complexity of interactions typical of a biological system.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping