PUBLICATION
Hepatoprotective Effect of Probiotic Lactobacillus rhamnosus GG Through the Modulation of Gut Permeability and Inflammasomes in a Model of Alcoholic Liver Disease in Zebrafish
- Authors
- Bruch-Bertani, J.P., Uribe-Cruz, C., Pasqualotto, A., Longo, L., Ayres, R., Bortolin Beskow, C., Luis Barth, A., Lima-Morales, D., Meurer, F., Tayguara, G., Guerreiro, S., da Silveira, T.R., Álvares-da-Silva, M.R., Dall'Alba, V.
- ID
- ZDB-PUB-190627-17
- Date
- 2019
- Source
- Journal of the American College of Nutrition 39(2): 163-170 (Journal)
- Registered Authors
- Keywords
- GG, alcoholic liver disease, inflammasomes, probiotics, zebrafish
- MeSH Terms
-
- Animals
- Disease Models, Animal
- Ethanol/administration & dosage
- Fatty Liver/therapy
- Gastrointestinal Microbiome/physiology
- Gene Expression/physiology
- Inflammasomes/genetics
- Inflammasomes/physiology*
- Intestinal Mucosa/metabolism*
- Lacticaseibacillus rhamnosus/growth & development
- Lacticaseibacillus rhamnosus/physiology*
- Lipid Metabolism/physiology
- Liver/metabolism
- Liver Diseases, Alcoholic/therapy*
- Permeability
- Probiotics/therapeutic use*
- Zebrafish*
- PubMed
- 31241423 Full text @ J Am Coll Nutr
Citation
Bruch-Bertani, J.P., Uribe-Cruz, C., Pasqualotto, A., Longo, L., Ayres, R., Bortolin Beskow, C., Luis Barth, A., Lima-Morales, D., Meurer, F., Tayguara, G., Guerreiro, S., da Silveira, T.R., Álvares-da-Silva, M.R., Dall'Alba, V. (2019) Hepatoprotective Effect of Probiotic Lactobacillus rhamnosus GG Through the Modulation of Gut Permeability and Inflammasomes in a Model of Alcoholic Liver Disease in Zebrafish. Journal of the American College of Nutrition. 39(2):163-170.
Abstract
Objective: Alcoholic liver disease (ALD) is among the leading causes of death from liver disease. Among the factors involved in its pathogenesis are inflammation and increased intestinal permeability. The aim of this study was to assess the effect of Lactobacillus rhamnosus GG (LGG) on hepatic lipid accumulation, activation of inflammasomes, and gut permeability markers in experimental model of ALD with zebrafish. Methods: An experiment was conducted to assess the effective LGG dose capable of promoting intestinal colonization. Animals were divided into three groups (n = 64/group): ethanol group (E), ethanol + probiotic group (EP), and control group (C). Groups E and EP were exposed to 0.5% ethanol concentration for 28 days. At the end of this period, animals were euthanized, and livers were collected for Oil Red staining and assessment of the inflammasome system. Intestines were collected for evaluation of gut permeability markers. Results: The dose of 1.55 × 106 UFC LGG/fish/d promoted intestinal colonization. Group EP presented lower hepatic lipid accumulation, lower il-1β expression, and higher cldn15a expression when compared to group E. Conclusions: Supplementation with LGG was protective for hepatic steatosis in ALD model. In addition, LGG influenced the modulation of the inflammatory response and markers of gut permeability, improving the gut barrier structure.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping