PUBLICATION
Impact of co-exposure to titanium dioxide nanoparticles and Pb on zebrafish embryos
- Authors
- Hu, S., Han, J., Yang, L., Li, S., Guo, Y., Zhou, B., Wu, H.
- ID
- ZDB-PUB-190614-17
- Date
- 2019
- Source
- Chemosphere 233: 579-589 (Journal)
- Registered Authors
- Guo, YongYong
- Keywords
- Bioavailability, Co-exposure, Pb, Titanium dioxide nanoparticles, Zebrafish embryos
- MeSH Terms
-
- Animals
- Biological Availability
- Embryo, Nonmammalian/drug effects
- Embryo, Nonmammalian/metabolism
- Embryo, Nonmammalian/physiology*
- Larva/metabolism
- Lead/toxicity*
- Metal Nanoparticles/toxicity*
- Metallothionein/metabolism
- Nanoparticles/toxicity
- Titanium/toxicity*
- Water Pollutants, Chemical/toxicity*
- Zebrafish/embryology*
- Zebrafish/metabolism
- PubMed
- 31195263 Full text @ Chemosphere
Citation
Hu, S., Han, J., Yang, L., Li, S., Guo, Y., Zhou, B., Wu, H. (2019) Impact of co-exposure to titanium dioxide nanoparticles and Pb on zebrafish embryos. Chemosphere. 233:579-589.
Abstract
Complex interactions have been established between nanoparticles (NPs) and heavy metals in real environments. Herein we used zebrafish embryos to investigate the influence of titanium dioxide NPs (n-TiO2) on the uptake, bioconcentration, and depuration, and toxicity of Pb. The formation of n-TiO2-Pb complexes was confirmed in an exposure suspension. An increase in Pb bioconcentration was observed in zebrafish embryos upon co-exposure to n-TiO2 and Pb; moreover, n-TiO2-Pb complexes could be found in the embryos, indicating the bioavailability of NPs. However, there was no difference in the depuration rates of Pb in the presence of n-TiO2. Metallothionein (MT) content was significantly increased upon exposure to Pb alone, and the content significantly increased even further upon co-exposure. A downregulation in the expression levels of the neurodevelopment-related genes gfap, syn2α, and elavl3 was observed in the embryos, and we also noted a reduction in the swimming speed of and the total distance traveled by the larvae. To summarize, our results indicate that n-TiO2 can act as an effective carrier of Pb to enhance its uptake, bioavailability, and toxicity in zebrafish embryos.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping