PUBLICATION
Redox Signaling via Lipid Peroxidation Regulates Retinal Progenitor Cell Differentiation
- Authors
- Albadri, S., Naso, F., Thauvin, M., Gauron, C., Parolin, C., Duroure, K., Vougny, J., Fiori, J., Boga, C., Vriz, S., Calonghi, N., Del Bene, F.
- ID
- ZDB-PUB-190611-3
- Date
- 2019
- Source
- Developmental Cell 50(1): 73-89.e6 (Journal)
- Registered Authors
- Albadri, Shahad, Del Bene, Filippo, Duroure, Karine, Gauron, Carole, Vriz, Sophie
- Keywords
- 9-hydroxystearic acid, H(2)O(2), HDAC1, lipid peroxidation, neuronal differentiation, redox signaling, retinal progenitor cells, stem cell metabolism, zebrafish
- MeSH Terms
-
- Histone Deacetylase 1/genetics
- Histone Deacetylase 1/metabolism
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- Neurogenesis*
- Zebrafish
- Oxidation-Reduction
- Cell Differentiation*
- Reactive Oxygen Species/metabolism*
- Stem Cells/cytology*
- Stem Cells/physiology
- Animals
- Cell Proliferation
- Retina/cytology*
- Retina/physiology
- Lipid Peroxidation*
- PubMed
- 31178398 Full text @ Dev. Cell
Citation
Albadri, S., Naso, F., Thauvin, M., Gauron, C., Parolin, C., Duroure, K., Vougny, J., Fiori, J., Boga, C., Vriz, S., Calonghi, N., Del Bene, F. (2019) Redox Signaling via Lipid Peroxidation Regulates Retinal Progenitor Cell Differentiation. Developmental Cell. 50(1):73-89.e6.
Abstract
Reactive oxygen species (ROS) and downstream products of lipid oxidation are emerging as important secondary messengers in tissue homeostasis. However, their regulation and mechanism of action remain poorly studied in vivo during normal development. Here, we reveal that the fine regulation of hydrogen peroxide (H2O2) levels by its scavenger Catalase to mediate the switch from proliferation to differentiation in retinal progenitor cells (RPCs) is crucial. We identify 9-hydroxystearic acid (9-HSA), an endogenous downstream lipid peroxidation product, as a mediator of this effect in the zebrafish retina. We show that the 9-HSA proliferative effect is due to the activation of Notch and Wnt pathways through the inhibition of the histone deacetylase 1. We show that the local and temporal manipulation of H2O2 levels in RPCs is sufficient to trigger their premature differentiation. We finally propose a mechanism that links H2O2 homeostasis and neuronal differentiation via the modulation of lipid peroxidation.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping