PUBLICATION
Visualizing primordial germ cell migration in embryos of rice field eel (Monopterus albus) using fluorescent protein tagged 3' untranslated regions of nanos3, dead end and vasa
- Authors
- Xiao, Q., Sun, Y., Liang, X., Zhang, L., Kommaly, O., Li, Z., Li, D.
- ID
- ZDB-PUB-190610-2
- Date
- 2019
- Source
- Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology 235: 62-69 (Journal)
- Registered Authors
- Keywords
- 3′ untranslated region, Dead end, Monopterus albus, Primordial germ cells, Vasa, nanos3
- MeSH Terms
-
- 3' Untranslated Regions
- Amino Acid Sequence
- Animals
- Cell Movement/genetics
- Cell Movement/physiology
- DEAD-box RNA Helicases/genetics
- DEAD-box RNA Helicases/metabolism
- Female
- Fish Proteins/genetics
- Fish Proteins/metabolism
- Germ Cells/cytology
- Germ Cells/metabolism*
- Luminescent Proteins/genetics
- Luminescent Proteins/metabolism
- Male
- RNA/genetics
- RNA, Messenger/genetics
- RNA-Binding Proteins/genetics
- RNA-Binding Proteins/metabolism
- Recombinant Fusion Proteins/genetics
- Recombinant Fusion Proteins/metabolism
- Sequence Homology, Amino Acid
- Smegmamorpha/embryology*
- Smegmamorpha/genetics
- Smegmamorpha/metabolism
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- PubMed
- 31176867 Full text @ Comp. Biochem. Physiol. B Biochem. Mol. Biol.
Citation
Xiao, Q., Sun, Y., Liang, X., Zhang, L., Kommaly, O., Li, Z., Li, D. (2019) Visualizing primordial germ cell migration in embryos of rice field eel (Monopterus albus) using fluorescent protein tagged 3' untranslated regions of nanos3, dead end and vasa. Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology. 235:62-69.
Abstract
In rice field eel (Monopterus albus), germ cell development in the developing gonad has been revealed in detail. However, it is unclear how primordial germ cells (PGCs) migrate to the somatic part of the gonad (genital ridge). This study visualized PGC migration by injecting a chimeric mRNA containing a fluorescent protein fused to the 3' untranslated region (3'UTR) of three different genes, nanos3 of zebrafish (Danio rerio) and dead end (dnd) and vasa of rice field eel. The mRNAs were injected either alone or in pairs into embryos at the one-cell stage. The results showed that mRNAs containing nanos3 and dnd 3'UTRs labeled PGCs over a wider time frame than those containing vasa 3'UTR, suggesting that nanos3 and dnd 3'UTRs are suitable for visualizing PGCs in rice field eel. Using this direct visualization method, the normal migration route of PGCs was observed from the 50%-epiboly stage to hatching stage for the first time, and the ectopic PGCs were also visualized during this period in rice field eel. These findings extend our knowledge of germ cell development, and lay a foundation for further research on the relationship between PGCs and sex differentiation, and on incubation conditions for embryos in rice field eel.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping