PUBLICATION
All-optical imaging and manipulation of whole-brain neuronal activities in behaving larval zebrafish
- Authors
- Jiao, Z.F., Shang, C.F., Wang, Y.F., Yang, Z., Yang, C., Li, F.N., Xie, J.Z., Pan, J.W., Fu, L., Du, J.L.
- ID
- ZDB-PUB-190509-4
- Date
- 2018
- Source
- Biomedical Optics Express 9: 6154-6169 (Journal)
- Registered Authors
- Du, Jiu Lin, Xie, Jin-Ze
- Keywords
- none
- MeSH Terms
- none
- PubMed
- 31065420 Full text @ Biomed. Opt. Express
Citation
Jiao, Z.F., Shang, C.F., Wang, Y.F., Yang, Z., Yang, C., Li, F.N., Xie, J.Z., Pan, J.W., Fu, L., Du, J.L. (2018) All-optical imaging and manipulation of whole-brain neuronal activities in behaving larval zebrafish. Biomedical Optics Express. 9:6154-6169.
Abstract
All-optical interrogation of population neuron activity is a promising approach to deciphering the neural circuit mechanisms supporting brain functions. However, this interrogation is currently limited to local brain areas. Here, we incorporate patterned photo-stimulation into light-sheet microscopy, allowing simultaneous targeted optogenetic manipulation and brain-wide monitoring of the neuronal activities of head-restrained behaving larval zebrafish. Using this system, we photo-stimulate arbitrarily selected neurons (regions as small as ~10-20 neurons in 3D) in zebrafish larvae with pan-neuronal expression of a spectrally separated calcium indicator, GCaMP6f, and an activity actuator, ChrimsonR, and observe downstream neural circuit activation and behavior generation. This approach allows us to dissect the causal role of neural circuits in brain functions and behavior generation.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping