PUBLICATION
Unravelling the mechanisms of PFOS toxicity by combining morphological and transcriptomic analyses in zebrafish embryos
- Authors
- Martínez, R., Navarro-Martín, L., Luccarelli, C., Codina, A.E., Raldúa, D., Barata, C., Tauler, R., Piña, B.
- ID
- ZDB-PUB-190426-9
- Date
- 2019
- Source
- The Science of the total environment 674: 462-471 (Journal)
- Registered Authors
- Piña, Benjamin, Raldúa, Demetrio
- Keywords
- ANOVA-PLS, Benchmark doses, Differentially expressed genes (DEGs), Endocrine disrupting chemicals, High-throughput sequencing, Immune system, Lipid disruption
- Datasets
- GEO:GSE125072
- MeSH Terms
-
- Alkanesulfonic Acids/toxicity*
- Animals
- Embryo, Nonmammalian/drug effects
- Embryo, Nonmammalian/physiology
- Fluorocarbons/toxicity*
- Gene Expression Profiling
- Water Pollutants, Chemical/toxicity*
- Zebrafish/embryology*
- PubMed
- 31022537 Full text @ Sci. Total Environ.
Citation
Martínez, R., Navarro-Martín, L., Luccarelli, C., Codina, A.E., Raldúa, D., Barata, C., Tauler, R., Piña, B. (2019) Unravelling the mechanisms of PFOS toxicity by combining morphological and transcriptomic analyses in zebrafish embryos. The Science of the total environment. 674:462-471.
Abstract
Exposure to PFOS (perfluorooctanesulfonate) has been related to toxic effects on lipid metabolism, immunological response, and different endocrine systems. We present here a transcriptomic analysis of zebrafish embryos exposed to different concentrations of PFOS (0.03-1.0 mg/L) from 48 to 120 hpf. No major survival or morphological alterations (swimming bladder inflation, kyphosis, eye separation and size…) were observed below the 1.0 mg/L mark. Conversely, we observed significant increase in transcripts related to lipid transport and metabolism even at the lowest used concentration. In addition, we observed a general decrease on transcripts related to natural immunity and defense again infections, which adds to the recent concerns about PFOS as immunotoxicant, particularly in humans. Derived PoD (Point of Departure) values for transcriptional changes (0.011 mg/L) were about 200-fold lower than the corresponding PoD values for morphometric effects (2.53 mg/L), and close to levels observed in human blood serum or bird eggs. Our data suggest that currently applicable tolerable levels of PFOS in commercial goods should be re-evaluated, taking into account its potential effects on lipid metabolism and the immune system.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping