PUBLICATION
Acute exposure to ustiloxin A affects growth and development of early life zebrafish, Danio rerio
- Authors
- Hu, Z., Dang, Y., Liu, C., Zhou, L., Liu, H.
- ID
- ZDB-PUB-190413-13
- Date
- 2019
- Source
- Chemosphere 226: 851-857 (Journal)
- Registered Authors
- Keywords
- Acute exposure, Developmental toxicity, Embryos, Rice false smut, Zebrefish, ustiloxin A
- MeSH Terms
-
- Animals
- Dose-Response Relationship, Drug
- Embryo, Nonmammalian/drug effects*
- Gene Expression Profiling
- Hypocreales
- Larva/drug effects*
- Larva/genetics
- Mycotoxins/toxicity
- Oryza/microbiology
- Peptides, Cyclic/chemistry
- Peptides, Cyclic/toxicity*
- Zebrafish/embryology
- Zebrafish/growth & development
- PubMed
- 30978596 Full text @ Chemosphere
Citation
Hu, Z., Dang, Y., Liu, C., Zhou, L., Liu, H. (2019) Acute exposure to ustiloxin A affects growth and development of early life zebrafish, Danio rerio. Chemosphere. 226:851-857.
Abstract
Ustiloxin A is a cyclopeptide mycotoxin originally isolated from rice false smut balls (FSBs) that formed in rice spikelets infected by the fungal pathogen Ustilaginoidea virens. Studies have shown that ustiloxin A was toxic to animals, but the toxicological evidence is still lacking. To reveal the negative influence of ustiloxin A on model organism, zebrafish were selected and exposed to ustiloxin A at concentrations of 0, 0.25, 2.5 or 25 μM from 2 h post-fertilization (hpf) to 144 hpf. The hatching rates of embryos in the 25 μM exposure group was 12.85% less than the control group at 96 hpf. Meanwhile, exposure to 0.25, 2.5 or 25 μM ustiloxin A resulted in a distinct dose-dependent increase in mortality rate of embryos at 96 hpf. We also found that exposed to ustiloxin A could cause some other damages on zebrafish larvae, such as growth delay and increased heart rate. In addition, the athletic behavior of zebrafish larvae exposed to ustiloxin A at 25 μM was dramatically different with that of control. Transcriptome sequencing showed that abundances of 339 transcripts (125 up-regulated and 214 down-regulated) were significantly altered in larvae exposed to 25 μM of ustiloxin A. Several of the crucial genes were validated by RT-qPCR. This is the first report on the toxicologic study of ustiloxins against model organism zebrafish. Results suggested that ustiloxins have become a potential danger for food security.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping