PUBLICATION
Rational design of a long-wavelength fluorescent probe for highly selective sensing carboxylesterase 1 in living systems
- Authors
- Tian, Z., Ding, L., Li, K., Song, Y., Dou, T., Hou, J., Tian, X., Feng, L., Ge, G., Cui, J.N.
- ID
- ZDB-PUB-190411-7
- Date
- 2019
- Source
- Analytical chemistry 91(9): 5638-5645 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Zebrafish
- Drug Design*
- Hydrolysis
- Carboxylesterase/chemistry
- Carboxylesterase/metabolism*
- Molecular Imaging
- Models, Molecular
- Boron Compounds/chemistry
- Fluorescent Dyes/chemistry*
- Protein Conformation
- Humans
- Animals
- Caco-2 Cells
- PubMed
- 30968686 Full text @ Anal. Chem.
Citation
Tian, Z., Ding, L., Li, K., Song, Y., Dou, T., Hou, J., Tian, X., Feng, L., Ge, G., Cui, J.N. (2019) Rational design of a long-wavelength fluorescent probe for highly selective sensing carboxylesterase 1 in living systems. Analytical chemistry. 91(9):5638-5645.
Abstract
Rational design of practical probes with excellent specificity and improved optical properties for a particular enzyme is always a big challenge. Herein, a practical and highly specific fluorescent probe for carboxylesterase 1 (CES1) was rationally designed using meso-carboxyl-BODIPY as the basic fluorophore based on the substrate preference and catalytic properties of CES1. Following molecular docking-based virtual screening combined with reaction phenotyping-based experimental screening, we found that MMB (probe 7) exhibited the optimal combination of sensitivity and specificity towards human CES1 in contrast to other ester derivatives. Under physiological conditions, MMB could be readily hydrolyzed by CES1 and released MCB, such biotransformation brought great changes in electronic properties at meso-position of the fluorophore and triggered a dramatic increase in fluorescence emission around 595 nm. Moreover, MMB was cell membrane permeable and was successfully applied to monitor the real activities of CES1 in various biological samples including living cells, tissue slices, organs and zebrafish. In summary, this study showed a good example for constructing specific fluorescent probe(s) for a target enzyme, and also provided a practical and sensitive tool for real-time sensing of CES1 activities in complicated biological samples. All these findings would strongly facilitate high-throughput screening of CES1 modulators and the studies on CES1-associated physiological and pathological processes.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping