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ZFIN ID: ZDB-PUB-190406-10
Reversible induction of mitophagy by an optogenetic bimodular system
D'Acunzo, P., Strappazzon, F., Caruana, I., Meneghetti, G., Di Rita, A., Simula, L., Weber, G., Del Bufalo, F., Dalla Valle, L., Campello, S., Locatelli, F., Cecconi, F.
Date: 2019
Source: Nature communications   10: 1533 (Journal)
Registered Authors: Dalla Valle, Luisa
Keywords: none
MeSH Terms:
  • Adaptor Proteins, Signal Transducing/metabolism
  • Adaptor Proteins, Signal Transducing/physiology*
  • Animals
  • HEK293 Cells
  • HeLa Cells
  • Humans
  • Lymphocytes/cytology
  • Mice
  • Mitochondria/metabolism
  • Mitophagy*
  • Optogenetics*
  • Zebrafish
PubMed: 30948710 Full text @ Nat. Commun.
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ABSTRACT
Autophagy-mediated degradation of mitochondria (mitophagy) is a key process in cellular quality control. Although mitophagy impairment is involved in several patho-physiological conditions, valuable methods to induce mitophagy with low toxicity in vivo are still lacking. Herein, we describe a new optogenetic tool to stimulate mitophagy, based on light-dependent recruitment of pro-autophagy protein AMBRA1 to mitochondrial surface. Upon illumination, AMBRA1-RFP-sspB is efficiently relocated from the cytosol to mitochondria, where it reversibly mediates mito-aggresome formation and reduction of mitochondrial mass. Finally, as a proof of concept of the biomedical relevance of this method, we induced mitophagy in an in vitro model of neurotoxicity, fully preventing cell death, as well as in human T lymphocytes and in zebrafish in vivo. Given the unique features of this tool, we think it may turn out to be very useful for a wide range of both therapeutic and research applications.
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